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Western Blot (WB) (Western blot analysis of lysates from HT-29 cells, using HNF4 alpha Antibody. The lane on the right is blocked with the synthesized peptide.)

Rabbit HNF4 alpha Polyclonal Antibody | anti-HNF4 alpha antibody

HNF4 alpha Antibody

Gene Names
HNF4A; TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; HNF4alpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Synonyms
HNF4 alpha; Polyclonal Antibody; HNF4 alpha Antibody; HNF-4; HNF-4-alpha; HNF4; HNF4A; Hepatocyte nuclear factor 4-alpha; NR2A1; TCF14; Transcription factor 14; Transcription factor HNF-4; anti-HNF4 alpha antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
HNF4 alpha Antibody detects endogenous levels of total HNF4 alpha protein.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
395
Applicable Applications for anti-HNF4 alpha antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
ELISA: 1:10000
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human HNF4 alpha.
Immunogen Range
280-329
Preparation and Storage
Stable at -20 degree C for at least 1 year.

Western Blot (WB)

(Western blot analysis of lysates from HT-29 cells, using HNF4 alpha Antibody. The lane on the right is blocked with the synthesized peptide.)

Western Blot (WB) (Western blot analysis of lysates from HT-29 cells, using HNF4 alpha Antibody. The lane on the right is blocked with the synthesized peptide.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52 kDa
NCBI Official Full Name
hepatocyte nuclear factor 4-alpha 2
NCBI Official Synonym Full Names
hepatocyte nuclear factor 4 alpha
NCBI Official Symbol
HNF4A
NCBI Official Synonym Symbols
TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; HNF4alpha
NCBI Protein Information
hepatocyte nuclear factor 4-alpha
UniProt Protein Name
Hepatocyte nuclear factor 4-alpha
UniProt Gene Name
HNF4A
UniProt Synonym Gene Names
HNF4; NR2A1; TCF14; HNF-4-alpha; TCF-14

NCBI Description

The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012]

Uniprot Description

Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine.

Research Articles on HNF4 alpha

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Product Notes

The HNF4 alpha hnf4a (Catalog #AAA9504160) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HNF4 alpha Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HNF4 alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500~1:1000 ELISA: 1:10000. Researchers should empirically determine the suitability of the HNF4 alpha hnf4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HNF4 alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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