Rabbit anti-Human FADD Polyclonal Antibody | anti-FADD antibody

FADD Antibody

Cat. Num. AAA9503537

• Gene Names: FADD; GIG3; MORT1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Synonyms: FADD; Polyclonal Antibody; FADD Antibody; FAS-associating death domain-containing protein; Mediator of receptor induced toxicity; MORT1; anti-FADD antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: FADD Antibody detects endogenous levels of total FADD protein.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 208

• Applicable Applications for anti-FADD antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:5000
• Target Modification: Unmodified/Total
• Immunogen: The antiserum was produced against synthesized peptide derived from human FADD.
• Immunogen Range: 159-208
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using FADD Antibody. The picture on the right is blocked with the synthesized peptide.)

Western Blot (WB)

(Western blot analysis of lysates from HeLa cells, treated with PMA 125ng/ml 30', using FADD Antibody. The lane on the right is blocked with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 4505229
• NCBI GeneID: 8772
• NCBI Accession #: NP_003815.1
• NCBI GenBank Nucleotide #: NM_003824.3
• UniProt Accession #: Q13158; Q14866; Q6IBR4
• Molecular Weight: 23 kDa
• NCBI Official Full Name: FAS-associated death domain protein
• NCBI Official Synonym Full Names: Fas associated via death domain
• NCBI Official Symbol: FADD
• NCBI Official Synonym Symbols: GIG3; MORT1
• NCBI Protein Information: FAS-associated death domain protein
• UniProt Protein Name: FAS-associated death domain protein
• Protein Family: Fas-associated death domain protein
• UniProt Gene Name: FADD
• UniProt Synonym Gene Names: MORT1

NCBI Description

The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development. [provided by RefSeq, Jul 2008]

Uniprot Description

Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis. Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling.