Immunofluorescence (IF)
(Immunofluorescence analysis of HUVEC cells, using NIFK (Phospho-Thr234) Antibody. The picture on the right is blocked with the phospho peptide.)
Rabbit anti-Human, Mouse NIFK Polyclonal Antibody | anti-NIFK antibody
NIFK (Phospho-Thr234) Antibody
Gene Names
NIFK; Nopp34; MKI67IP
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunofluorescence, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Synonyms
NIFK; Polyclonal Antibody; NIFK (Phospho-Thr234) Antibody; MKI67IP; Nucleolar protein NIFK; RNA binding protein; anti-NIFK antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
NIFK (Phospho-Thr234) Antibody detects endogenous levels of NIFK only when phosphorylated at Thr234.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
293
Applicable Applications for anti-NIFK antibody
Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:40000
IF: 1:100~1:500
ELISA: 1:40000
Target Modification
Phospho
Modification Sites
H:T234; M:T257
Immunogen
The antiserum was produced against synthesized peptide derived from human NIFK around the phosphorylation site of Thr234.
Immunogen Range
200-249
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Immunofluorescence (IF)
(Immunofluorescence analysis of HUVEC cells, using NIFK (Phospho-Thr234) Antibody. The picture on the right is blocked with the phospho peptide.)
Immunofluorescence (IF)
(Immunofluorescence analysis of HUVEC cells, using NIFK (Phospho-Thr234) Antibody. The picture on the right is blocked with the phospho peptide.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human brain, using NIFK (Phospho-Thr234) Antibody. The picture on the right is blocked with the phospho peptide.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human brain, using NIFK (Phospho-Thr234) Antibody. The picture on the right is blocked with the phospho peptide.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34 kDa
NCBI Official Full Name
MKI67 FHA domain-interacting nucleolar phosphoprotein
NCBI Official Synonym Full Names
nucleolar protein interacting with the FHA domain of MKI67
NCBI Official Symbol
NIFK
NCBI Official Synonym Symbols
Nopp34; MKI67IP
NCBI Protein Information
MKI67 FHA domain-interacting nucleolar phosphoprotein
UniProt Protein Name
MKI67 FHA domain-interacting nucleolar phosphoprotein
Protein Family
UniProt Gene Name
NIFK
UniProt Synonym Gene Names
MKI67IP; NOPP34; hNIFK
NCBI Description
This gene encodes a protein that interacts with the forkhead-associated domain of the Ki-67 antigen. The encoded protein may bind RNA and may play a role in mitosis and cell cycle progression. Multiple pseudogenes exist on chromosomes 5, 10, 12, 15, and 19.[provided by RefSeq, Jan 2009]
Research Articles on NIFK
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Product Notes
The NIFK nifk (Catalog #AAA9502869) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NIFK (Phospho-Thr234) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NIFK can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000. Researchers should empirically determine the suitability of the NIFK nifk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NIFK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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