Human Osteopontin ELISA Kit | OPN elisa kit

Osteopontin (Human) OmniKine ELISA Kit

Cat. Num. AAA9502065

• Gene Names: SPP1; OPN; BNSP; BSPI; ETA-1
• Reactivity: Human
• Synonyms: Osteopontin; Osteopontin (Human) OmniKine ELISA Kit; Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin; OPN elisa kit

• Reactivity: Human
• Specificity: The OmniKine Human Osteopontin ELISA is capable of recognizing both recombinant and naturally produced Human Osteopontin proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.; Human: MMP-3
• Form/Format: 12 x 8-Well Microstrips
• Sequence Length: 300

• Samples: Plasma, serum, lysate
• Assay Type: Sandwich ELISA
• Detection Range: 63-4000 pg/ml
• Sensitivity: 63-4000 pg/ml
• Detection Method: Colorimetric 450 nm
• Subtype: None
• Preparation and Storage: Store at 4 degree C for 6 months.

Testing Data

(The OmniKine Human Osteopontin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Osteopontin proteins within the range of 63-4000 pg/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 4759166
• NCBI GeneID: 6696
• NCBI Accession #: NP_000573.1
• NCBI GenBank Nucleotide #: NM_000582.2
• UniProt Accession #: P10451; Q15681; Q15682; Q15683; Q4W597; Q567T5; Q8NBK2; Q96IZ1; B2RDA1
• Molecular Weight: 33,843 Da
• NCBI Official Full Name: osteopontin isoform OPN-b
• NCBI Official Synonym Full Names: secreted phosphoprotein 1
• NCBI Official Symbol: SPP1
• NCBI Official Synonym Symbols: OPN; BNSP; BSPI; ETA-1
• NCBI Protein Information: osteopontin
• UniProt Protein Name: Osteopontin
• Protein Family: Osteopontin
• UniProt Gene Name: SPP1
• UniProt Synonym Gene Names: BNSP; OPN; SPP-1

NCBI Description

The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

osteopontin: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Belongs to the osteopontin family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q22.1

Cellular Component: cell projection; endoplasmic reticulum lumen; extracellular exosome; extracellular region; extracellular space; Golgi apparatus; perinuclear region of cytoplasm

Molecular Function: cytokine activity; extracellular matrix binding; protein binding

Biological Process: androgen catabolic process; biomineral formation; cell adhesion; cellular protein metabolic process; cellular response to testosterone stimulus; decidualization; embryo implantation; extracellular matrix disassembly; extracellular matrix organization; inflammatory response; negative regulation of collateral sprouting of intact axon in response to injury; osteoblast differentiation; positive regulation of bone resorption; positive regulation of estradiol secretion; positive regulation of transcription, DNA-templated; post-translational protein modification; regulation of receptor activity; response to steroid hormone; response to vitamin D