Human E-Cadherin ELISA Kit | CDH1 elisa kit

E-Cadherin (Human) OmniKine ELISA Kit

Cat. Num. AAA9501943

• Gene Names: CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324
• Reactivity: Human
• Synonyms: E-Cadherin; E-Cadherin (Human) OmniKine ELISA Kit; CAM 120/80; Epithelial cadherin; Uvomorulin; CD324; CDHE; UVO; CDH1 elisa kit

• Reactivity: Human
• Specificity: The OmniKine Human E-Cadherin ELISA is capable of recognizing both recombinant and naturally produced Human E-Cadherin proteins. The antigens listed below were tested at 60 ng/ml and exhibited less than 2% cross reactivity.; Murine: E-Cadherin; The antigens listed below were tested at 120 ng/ml and did not exhibit significant cross reactivity or interference.; Human: N-Cadherin, P-Cadherin, VE-Cadherin; Murine: P-Cadherin
• Form/Format: 12 x 8-Well Microstrips
• Sequence Length: 821

• Samples: Plasma, serum, lysate
• Assay Type: Sandwich ELISA
• Detection Range: 188-12000 pg/ml
• Sensitivity: 188-12000 pg/ml
• Detection Method: Colorimetric 450 nm
• Subtype: None
• Preparation and Storage: Store at 4 degree C for 6 months.

Testing Data

(The OmniKine Human E-Cadherin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human E-Cadherin proteins within the range of 188-12000 pg/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 953768348
• NCBI GeneID: 999
• NCBI Accession #: NP_001304113.1
• NCBI GenBank Nucleotide #: NM_001317184.1
• UniProt Accession #: P12830; Q13799; Q14216; Q15855; Q16194; Q4PJ14; Q9UII8; A8K1U7
• Molecular Weight: 90,942 Da
• NCBI Official Full Name: cadherin-1 isoform 2
• NCBI Official Synonym Full Names: cadherin 1
• NCBI Official Symbol: CDH1
• NCBI Official Synonym Symbols: UVO; CDHE; ECAD; LCAM; Arc-1; BCDS1; CD324
• NCBI Protein Information: cadherin-1
• UniProt Protein Name: Cadherin-1
• Protein Family: Cadherin
• UniProt Gene Name: CDH1
• UniProt Synonym Gene Names: CDHE; UVO; E-cadherin

NCBI Description

This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015]

Uniprot Description

CDH1: a single-pass type I membrane protein, and calcium dependent cell adhesion proteins. It is a ligand for integrin alpha-E/beta-7, and it colocalizes with DLG7 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Defects in CDH1 are involved in dysfunction of the cell-cell adhesion system, triggering cancer invasion (gastric, breast, ovary, endometrium and thyroid) and metastasis. Has a potent invasive suppressor role.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: actin cytoskeleton; apical junction complex; catenin complex; cell junction; cell-cell adherens junction; cortical actin cytoskeleton; cytoplasmic side of plasma membrane; endosome; extracellular exosome; extracellular region; flotillin complex; integral component of membrane; lamellipodium; lateral plasma membrane; perinuclear region of cytoplasm; plasma membrane; trans-Golgi network

Molecular Function: ankyrin binding; beta-catenin binding; cadherin binding; calcium ion binding; cell adhesion molecule binding; gamma-catenin binding; GTPase activating protein binding; identical protein binding; protein binding

Biological Process: cellular response to indole-3-methanol; cellular response to lithium ion; extracellular matrix disassembly; extracellular matrix organization; homophilic cell adhesion; negative regulation of cell-cell adhesion; neuron projection development; pituitary gland development; positive regulation of transcription factor import into nucleus; positive regulation of transcription, DNA-templated; protein localization to plasma membrane; response to drug; response to toxin; synaptogenesis

Disease: Blepharocheilodontic Syndrome 1; Breast Cancer; Endometrial Cancer; Gastric Cancer, Hereditary Diffuse; Ovarian Cancer; Prostate Cancer