Human Arrestin 1 ELISA Kit | ARRB1 elisa kit
Phospho-Arrestin 1 (Ser412) Colorimetric Cell-Based ELISA Kit
NCBI and Uniprot Product Information
NCBI Description
Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 1 is a cytosolic protein and acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Besides the central nervous system, it is expressed at high levels in peripheral blood leukocytes, and thus the BARK/beta-arrestin system is believed to play a major role in regulating receptor-mediated immune functions. Alternatively spliced transcripts encoding different isoforms of arrestin beta 1 have been described. [provided by RefSeq, Jan 2011]
Uniprot Description
ARRB1: regulates G-protein coupled receptors (GPCR) signaling by mediating both receptor desensitization and resensitization processes. Binds to GRK-phosphorylated receptor and sterically preclude its coupling to the cognate G- protein; the binding appears to require receptor determinants exposed only in the active receptor conformation. Targets many receptors for internalization by acting as endocytic adapters (CLASPs, clathrin-associated sorting proteins). Internalized arrestin-receptor complexes traffic to intracellular endosomes, where they remain uncoupled from G-proteins. Two different modes of arrestin-mediated internalization occur. Beta-arrestins function as multivalent adapter proteins that can switch the GPCR from a G-protein signaling mode that transmits short-lived signals from the plasma membrane via small molecule second messengers and ion channels to a beta-arrestin signaling mode that transmits a distinct set of signals that are initiated as the receptor internalizes and transits the intracellular compartment. Also involved in regulation of receptors other than GPCRs. Involved in Toll-like receptor and IL-1 receptor signaling through the interaction with TRAF6 which prevents TRAF6 autoubiquitination and oligomerization required for activation of NF-kappa-B and JUN. Binds phosphoinositides. Binds inositolhexakisphosphate (InsP6). Involved in IL8-mediated granule release in neutrophils. Interacts with phosphorylated ADRB2 and CHRM2. Interacts with SRC (via the SH3 domain and the protein kinase domain); the interaction is independent of the phosphorylation state of SRC C-terminus. Interacts with RAF1, CHUK, IKBKB and Nik. Interacts with DVL1 and DVL2; the interaction is enhanced by DVL phosphorylation. Interacts with IGF1R. Belongs to the arrestin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 11q13.4
Cellular Component: basolateral plasma membrane; chromatin; clathrin-coated pit; cytoplasm; cytoplasmic vesicle; cytoplasmic vesicle membrane; cytosol; dendritic spine; Golgi membrane; heterotrimeric G-protein complex; lysosomal membrane; nuclear body; nucleoplasm; nucleus; plasma membrane; postsynaptic density; postsynaptic membrane; pseudopodium
Molecular Function: alpha-1A adrenergic receptor binding; alpha-1B adrenergic receptor binding; angiotensin receptor binding; caspase inhibitor activity; enzyme inhibitor activity; estrogen receptor binding; follicle-stimulating hormone receptor binding; GTPase activator activity; histone acetyltransferase activity; insulin-like growth factor receptor binding; ion channel binding; mitogen-activated protein kinase kinase binding; protein binding; protein phosphorylated amino acid binding; transcription factor binding; transcription regulatory region DNA binding; ubiquitin protein ligase binding; V2 vasopressin receptor binding
Biological Process: activation of MAPK activity; apoptosis; follicle-stimulating hormone signaling pathway; G-protein coupled receptor internalization; G-protein coupled receptor signaling pathway; negative regulation of cysteine-type endopeptidase activity involved in apoptotic process; negative regulation of ERK1 and ERK2 cascade; negative regulation of interleukin-6 production; negative regulation of interleukin-8 production; negative regulation of NF-kappaB transcription factor activity; negative regulation of Notch signaling pathway; negative regulation of protein ubiquitination; phototransduction; platelet activation; positive regulation of cysteine-type endopeptidase activity involved in apoptotic process; positive regulation of ERK1 and ERK2 cascade; positive regulation of GTPase activity; positive regulation of histone acetylation; positive regulation of histone H4 acetylation; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein binding; positive regulation of protein phosphorylation; positive regulation of protein ubiquitination; positive regulation of receptor internalization; positive regulation of Rho protein signal transduction; positive regulation of smooth muscle cell apoptotic process; positive regulation of transcription from RNA polymerase II promoter; proteasomal ubiquitin-dependent protein catabolic process; protein transport; protein ubiquitination; response to drug; stress fiber assembly; transcription from RNA polymerase II promoter; ubiquitin-dependent protein catabolic process
Research Articles on ARRB1
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Product Notes
The Human ARRB1 arrb1 (Catalog #AAA9501088) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9501088 ELISA Kit recognizes Human ARRB1. It is sometimes possible for the material contained within the vial of "Arrestin 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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