Sulfonamides (SAs) residue ELISA Kit | LTA elisa kit

Sulfonamides (SAs) residue ELISA Kit

Cat. Num. AAA947871

• Gene Names: LTA; TNLG1E; TNF-beta
• Synonyms: Sulfonamides (SAs) residue; Sulfonamides (SAs) residue ELISA Kit; LTA elisa kit

• Sequence Length: 197

• Samples: Milk, tissue (chicken, pork, beef, liver), swine urine, eggs
• Assay Type: Competitive
• Detection Range: 1 ppb -81 ppb (for swine urine); 10 ppb (for milk, tissue, eggs)
• Sensitivity: 1ppb
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<10%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Related Product Information for LTA elisa kit

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with total Sulfonamides antigen. Standards or samples are added to the appropriate microtiter plate wells with total Sulfonamides specific antibody and Horseradish Peroxidase (HRP) conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated total Sulfonamides and total Sulfonamides in standards or samples with the total Sulfonamides special antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Sulfonamides residue in the standards or samples. The color development is stopped and the intensity of the color is measured.

Background/Introduction: Sulfonamides are widely used in animal industry and play an important role in controlling and treatment livestock and poultry diseases. Sulfonamides have become a threat for human health and affected the export of animal derived food due to Sulfonamides residue for abuse, not abiding by withdrawal time. Sulfonamides residue limit is 100 ?g/kg according to No.235 document of the Ministry of Agriculture of the People's Republic of China. The method of instrumental analytical is major to detect Sulfonamides residue, but it needs expensive instruments, professional operators and complex pre-treatment; While the method of enzyme linked immunoassay has advantages with simple, rapid, high sensitivity, good specificity and low cost. This kit is a detection product developed based on ELISA technology, with operation time as short as 50 min and a sensitivity of 1 ppb, and linear range from 1 ppb to 81 ppb.

NCBI and Uniprot Product Information

• NCBI GI #: 283837810
• NCBI GeneID: 100328931
• NCBI Accession #: NP_001164590.1
• NCBI GenBank Nucleotide #: NM_001171119.1
• Molecular Weight: 21,126 Da
• NCBI Official Full Name: lymphotoxin-alpha
• NCBI Official Symbol: LTA
• NCBI Official Synonym Symbols: TNLG1E; TNF-beta
• NCBI Protein Information: lymphotoxin-alpha
• UniProt Protein Name: Lymphotoxin-alpha
• Protein Family: Lymphotoxin
• UniProt Gene Name: LTA
• UniProt Synonym Gene Names: TNFB; TNFSF1; LT-alpha
• UniProt Entry Name: TNFB_RABIT

Uniprot Description

Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo.