Rabbit NLRP3 Polyclonal Antibody | anti-NLRP3 antibody

NLRP3 Polyclonal Antibody

Cat. Num. AAA9457640

• Gene Names: NLRP3; AII; AVP; FCU; MWS; FCAS; CIAS1; NALP3; C1orf7; CLR1.1; PYPAF1; AGTAVPRL; FLJ95925
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity purification
• Synonyms: NLRP3; Polyclonal Antibody; NLRP3 Polyclonal Antibody; AGTAVPRL; AII; AVP; C1orf7; CIAS1; CLR1.1; FCAS; FCAS1; FCU; MWS; NALP3; PYPAF1; anti-NLRP3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH7.4, 150mM NaCl, 0.02% sodium azide and 239% glycerol.; PBS with 0.02% sodium azide, 50% glycerol, pH7.192
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-NLRP3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:1000-1:3000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: A synthetic peptide of human NLRP3 (NP_001120934.1)
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Immunofluorescence (IF)

(Immunofluorescence analysis of Raw264.7 cells using NLRP3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

Immunofluorescence (IF)

(Immunofluorescence analysis of Raw264.7 cells using NLRP3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

Immunofluorescence (IF)

(Immunofluorescence analysis of rat lung cells using NLRP3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat lung using NLRP3 antibody at dilution of 1:100 .)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human appendix using NLRP3 antibody at dilution of 1:100 .)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse spleen using NLRP3 antibody at dilution of 1:100 .)

Western Blot (WB)

(Western blot analysis of extracts of U-937 cells, using NLRP3 antibody at 1:1000 dilution.)

Related Product Information for anti-NLRP3 antibody

This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid.

Product Categories/Family for anti-NLRP3 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 188536002
• NCBI GeneID: 114548
• NCBI Accession #: NP_001120934.1
• NCBI GenBank Nucleotide #: NM_001127462.2
• UniProt Accession #: Q96P20; Q65Z67
• Molecular Weight: 6,129 Da
• NCBI Official Full Name: NACHT, LRR and PYD domains-containing protein 3 isoform d
• NCBI Official Synonym Full Names: NLR family, pyrin domain containing 3
• NCBI Official Symbol: NLRP3
• NCBI Official Synonym Symbols: AII; AVP; FCU; MWS; FCAS; CIAS1; NALP3; C1orf7; CLR1.1; PYPAF1; AGTAVPRL; FLJ95925
• NCBI Protein Information: NACHT, LRR and PYD domains-containing protein 3; cryopyrin; OTTHUMP00000038408; OTTHUMP00000038409; OTTHUMP00000038410; OTTHUMP00000038411; OTTHUMP00000230825; caterpiller protein 1.1; PYRIN-containing APAF1-like protein 1; NACHT, LRR and PYD containing p
• UniProt Protein Name: NACHT, LRR and PYD domains-containing protein 3
• Protein Family: NACHT, LRR and PYD domains-containing protein
• UniProt Gene Name: NLRP3
• UniProt Synonym Gene Names: C1orf7; CIAS1; NALP3; PYPAF1; CLR1.1
• UniProt Entry Name: NALP3_HUMAN

Uniprot Description

NLRP3: May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1); also known as familial cold urticaria. FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold. Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS); also known as urticaria-deafness-amyloidosis syndrome. MWS is a hereditary periodic fever syndrome characterized by fever, chronic recurrent urticaria, arthralgias, progressive sensorineural deafness, and reactive renal amyloidosis. The disease may be severe if generalized amyloidosis occurs. Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA); also known as neonatal onset multisystem inflammatory disease (NOMID). CINCA is a rare congenital inflammatory disorder characterized by a triad of neonatal onset of cutaneous symptoms, chronic meningitis and joint manifestations with recurrent fever and inflammation. Belongs to the NLRP family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 1q44

Cellular Component: cytoplasm; cytosol

Molecular Function: protein binding; peptidoglycan binding; ATP binding

Biological Process: caspase activation; apoptosis; negative regulation of acute inflammatory response; positive regulation of caspase activity; interleukin-1 beta production; defense response; negative regulation of NF-kappaB import into nucleus; positive regulation of interleukin-1 beta secretion; signal transduction; protein oligomerization; activation of NF-kappaB transcription factor; negative regulation of interleukin-1 beta secretion; inhibition of NF-kappaB transcription factor; negative regulation of inflammatory response; interleukin-18 production; innate immune response; interleukin-1 secretion; inflammatory response; detection of biotic stimulus; defense response to virus

Disease: Cinca Syndrome; Familial Cold Autoinflammatory Syndrome 1; Muckle-wells Syndrome