Mouse Peroxisome Proliferators Activated Receptor alpha ELISA Kit | PPARalpha elisa kit

Mouse Peroxisome Proliferators Activated Receptor alpha ELISA Kit

Cat. Num. AAA94563

• Gene Names: PPARA; PPAR; NR1C1; hPPAR; PPARalpha
• Reactivity: Mouse
• Synonyms: Peroxisome Proliferators Activated Receptor alpha; N/A; Mouse Peroxisome Proliferators Activated Receptor alpha ELISA Kit; Mouse Peroxisome Proliferators Activated Receptor a ELISA Kit; PPARalpha elisa kit

• Reactivity: Mouse
• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Sensitivity: 1.0 pg/mL.

• Intended Uses: This PPARalpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse PPARalpha. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for PPARalpha elisa kit

Principle of the assay: PPARalpha ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PPARalpha. Standards or samples are then added to the microtiter plate wells and PPARalpha if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PPARalpha present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PPARalpha are added to each well to "sandwich" the PPARalpha immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PPARalpha and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PPARalpha concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 50348666
• NCBI GeneID: 5465
• NCBI Accession #: NP_001001928.1
• NCBI GenBank Nucleotide #: NM_001001928.2
• Molecular Weight: 18,942 Da
• NCBI Official Full Name: peroxisome proliferator-activated receptor alpha
• NCBI Official Synonym Full Names: peroxisome proliferator-activated receptor alpha
• NCBI Official Symbol: PPARA
• NCBI Official Synonym Symbols: PPAR; NR1C1; hPPAR; PPARalpha
• NCBI Protein Information: peroxisome proliferator-activated receptor alpha; PPAR-alpha; nuclear receptor subfamily 1 group C member 1; peroxisome proliferative activated receptor, alpha; peroxisome proliferator-activated nuclear receptor alpha variant 3
• UniProt Protein Name: Peroxisome proliferator-activated receptor alpha
• UniProt Gene Name: PPARA
• UniProt Synonym Gene Names: NR1C1; PPAR; PPAR-alpha
• UniProt Entry Name: PPARA_HUMAN