Human ephrin-A1 ELISA Kit | EFNA1 elisa kit

Human Ephrin-A1, EFNA1 ELISA Kit

Cat. Num. AAA945015

• Gene Names: EFNA1; B61; EFL1; ECKLG; EPLG1; LERK1; LERK-1; TNFAIP4
• Reactivity: Human
• Synonyms: ephrin-A1; Human Ephrin-A1; EFNA1 ELISA Kit; Human Ephrin-A1 (EFNA1) ELISA kit; B61; ECKLG; EFL1; EPLG1; LERK1; TNFAIP4; eph-related receptor tyrosine kinase ligand 1; ephrin A1; immediate early response protein B61; ligand of eph-related kinase 1; tumor necrosis factor; alpha-indu; EFNA1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human EFNA1. No significant cross-reactivity or interference between human EFNA1 and analogues was observed.
• Sequence Length: 205

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 0.156 ng/ml -10 ng/ml.
• Sensitivity: The minimum detectable dose of human EFNA1 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EFNA1 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EFNA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EFNA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EFNA1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EFNA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 33359682
• NCBI GeneID: 1942
• NCBI Accession #: NP_004419.2
• NCBI GenBank Nucleotide #: NM_004428.2
• UniProt Accession #: P20827; Q5SR60; Q5SR61; Q6I9T9; Q8N578; D3DV86
• Molecular Weight: 23,787 Da
• NCBI Official Full Name: ephrin-A1 isoform a
• NCBI Official Synonym Full Names: ephrin-A1
• NCBI Official Symbol: EFNA1
• NCBI Official Synonym Symbols: B61; EFL1; ECKLG; EPLG1; LERK1; LERK-1; TNFAIP4
• NCBI Protein Information: ephrin-A1; TNF alpha-induced protein 4; ligand of eph-related kinase 1; immediate early response protein B61; eph-related receptor tyrosine kinase ligand 1; tumor necrosis factor alpha-induced protein 4; tumor necrosis factor, alpha-induced protein 4
• UniProt Protein Name: Ephrin-A1
• Protein Family: Ephrin
• UniProt Gene Name: EFNA1
• UniProt Synonym Gene Names: EPLG1; LERK1; TNFAIP4; LERK-1; TNF alpha-induced protein 4
• UniProt Entry Name: EFNA1_HUMAN

NCBI Description

This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNA class ephrin which binds to the EPHA2, EPHA4, EPHA5, EPHA6, and EPHA7 receptors. Two transcript variants that encode different isoforms were identified through sequence analysis. [provided by RefSeq, Jul 2008]

Uniprot Description

EFNA1: Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Plays an important role in angiogenesis and tumor neovascularization. The recruitment of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cone and regulates dendritic spine morphogenesis. Belongs to the ephrin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Cell development/differentiation; Tumor suppressor; Ligand, receptor tyrosine kinase

Chromosomal Location of Human Ortholog: 1q21-q22

Cellular Component: anchored to plasma membrane; integral to plasma membrane; plasma membrane; extracellular region

Molecular Function: protein binding; ephrin receptor binding; receptor binding

Biological Process: axon guidance; cell migration; activation of MAPK activity; notochord formation; negative regulation of transcription from RNA polymerase II promoter; regulation of blood vessel endothelial cell migration; regulation of axonogenesis; regulation of angiogenesis; positive regulation of peptidyl-tyrosine phosphorylation; regulation of cell adhesion mediated by integrin; cell-cell signaling; ephrin receptor signaling pathway; angiogenesis; regulation of peptidyl-tyrosine phosphorylation