Human plexin C1 ELISA Kit | PLXNC1 elisa kit

Human Plexin-C1, PLXNC1 ELISA Kit

Cat. Num. AAA944227

• Gene Names: PLXNC1; CD232; VESPR; PLXN-C1
• Reactivity: Human
• Synonyms: plexin C1; Human Plexin-C1; PLXNC1 ELISA Kit; Human Plexin-C1 (PLXNC1) ELISA kit; CD232; PLXN-C1; VESPR; plexin (semaphorin receptor) ; receptor for virally-encoded semaphorin; PLXNC1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human PLXNC1. No significant cross-reactivity or interference between human PLXNC1 and analogues was observed.
• Sequence Length: 1,568

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 0.312 ng/ml -20 ng/ml.
• Sensitivity: The minimum detectable dose of human PLXNC1 is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PLXNC1 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PLXNC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLXNC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLXNC1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLXNC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 5032223
• NCBI GeneID: 10154
• NCBI Accession #: NP_005752.1
• NCBI GenBank Nucleotide #: NM_005761.2
• UniProt Accession #: O60486; Q59H25
• Molecular Weight: 175,742 Da
• NCBI Official Full Name: plexin-C1
• NCBI Official Synonym Full Names: plexin C1
• NCBI Official Symbol: PLXNC1
• NCBI Official Synonym Symbols: CD232; VESPR; PLXN-C1
• NCBI Protein Information: plexin-C1; plexin (semaphorin receptor); receptor for viral semaphorin protein; receptor for virally-encoded semaphorin; virus-encoded semaphorin protein receptor
• UniProt Protein Name: Plexin-C1
• Protein Family: Plexin
• UniProt Gene Name: PLXNC1
• UniProt Synonym Gene Names: VESPR
• UniProt Entry Name: PLXC1_HUMAN

NCBI Description

This gene encodes a member of the plexin family. Plexins are transmembrane receptors for semaphorins, a large family of proteins that regulate axon guidance, cell motility and migration, and the immune response. The encoded protein and its ligand regulate melanocyte adhesion, and viral semaphorins may modulate the immune response by binding to this receptor. The encoded protein may be a tumor suppressor protein for melanoma. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

PLXNC1: Receptor for SEMA7A, for smallpox semaphorin A39R, vaccinia virus semaphorin A39R and for herpesvirus Sema protein. Binding of semaphorins triggers cellular responses leading to the rearrangement of the cytoskeleton and to secretion of IL6 and IL8. Belongs to the plexin family.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12q23.3

Cellular Component: membrane; plasma membrane; integral to membrane; intracellular

Molecular Function: protein binding; receptor activity; receptor binding

Biological Process: axon guidance; signal transduction; cell adhesion