Human carboxypeptidase E ELISA Kit | CPE elisa kit

Human Carboxypeptidase E, CPE ELISA Kit

Cat. Num. AAA944061

• Reactivity: Human
• Synonyms: carboxypeptidase E; Human Carboxypeptidase E; CPE ELISA Kit; Human Carboxypeptidase E (CPE) ELISA kit; CPE elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human CPE. No significant cross-reactivity or interference between Human CPE and analogues was observed.
• Sequence Length: 476

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 39.06 pg/ml-2500 pg/ml
• Sensitivity: 9.76 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CPE elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CPE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CPE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CPE is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CPE bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4503009
• NCBI GeneID: 1363
• NCBI Accession #: NP_001864.1
• NCBI GenBank Nucleotide #: NM_001873.2
• UniProt Accession #: P16870; Q9UIU9; A8K4N1; B3KR42; B4DFN4; D3DP33
• Molecular Weight: 53,151 Da
• NCBI Official Full Name: carboxypeptidase E preproprotein
• NCBI Official Synonym Full Names: carboxypeptidase E
• NCBI Official Symbol: CPE
• NCBI Protein Information: carboxypeptidase E; CPH; carboxypeptidase H; enkephalin convertase; prohormone-processing carboxypeptidase; insulin granule-associated carboxypeptidase; cobalt-stimulated chromaffin granule carboxypeptidase
• UniProt Protein Name: Carboxypeptidase E
• Protein Family: Carboxypeptidase
• UniProt Gene Name: CPE
• UniProt Synonym Gene Names: CPE; CPH
• UniProt Entry Name: CBPE_HUMAN

NCBI Description

This gene encodes a carboxypeptidase that cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. It is a peripheral membrane protein. The protein specifically binds regulated secretory pathway proteins, including prohormones, but not constitutively secreted proteins. Mutations in this gene are implicated in type II diabetes. [provided by RefSeq, Jul 2008]

Uniprot Description

CPE: Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.17.10; Secreted; Secreted, signal peptide; Protease

Chromosomal Location of Human Ortholog: 4q32.3

Cellular Component: Golgi apparatus; secretory granule membrane; plasma membrane; nucleus

Molecular Function: carboxypeptidase activity; zinc ion binding; neurexin binding; cell adhesion molecule binding; metallocarboxypeptidase activity

Biological Process: insulin processing; cellular protein metabolic process; neuropeptide signaling pathway; metabolic process; protein modification process; proteolysis