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Western Blot (WB)
(Western blot analysis of PCK2 on human kidney tissue (1) and MCF-7 cell (2) lysate using anti-PCK2 antibody at 1/500 dilution.)
Rabbit PCK2 Monoclonal Antibody | anti-PCK2 antibody
PCK2 Antibody
Gene Names
PCK2; PEPCK; PEPCK2; PEPCK-M
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
PCK2; Monoclonal Antibody; PCK2 Antibody; EC 4.1.1.32 antibody; GTP mitochondrial precursor antibody; HGNC:8725 antibody; mitochondrial antibody; Mitochondrial phosphoenolpyruvate carboxykinase 2 antibody; OTTHUMP00000164700 antibody; PCK2 antibody; PCKGM_HUMAN antibody; PE antibody; PEP carboxykinase antibody; PEPCK antibody; PEPCK deficiency mitochondrial antibody; PEPCK M antibody; PEPCK-M antibody; PEPCK2 antibody; Phosphoenolpyruvate carboxykinase [GTP] antibody; Phosphoenolpyruvate carboxykinase 2 (mitochondrial) antibody; Phosphoenolpyruvate carboxykinase 2 mitochondrial antibody; Phosphoenolpyruvate carboxylase antibody; Phosphopyruvate carboxylase antibody; anti-PCK2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JB52-39
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-PCK2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF),Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
ICC: 1:50-1:100
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
ICC: 1:50-1:100
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western blot analysis of PCK2 on human kidney tissue (1) and MCF-7 cell (2) lysate using anti-PCK2 antibody at 1/500 dilution.)
Western Blot (WB)
(Western blot analysis of PCK2 on human kidney tissue (1) and MCF-7 cell (2) lysate using anti-PCK2 antibody at 1/500 dilution.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PCK2 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PCK2 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PCK2 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PCK2 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PCK2 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PCK2 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-PCK2 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-PCK2 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PCK2 antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PCK2 antibody. Counter stained with hematoxylin.)
Immunocytochemistry (ICC)
(ICC staining PCK2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining PCK2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining PCK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining PCK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of MCF-7 cells with PCK2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of MCF-7 cells with PCK2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Related Product Information for anti-PCK2 antibody
Normal adjustment to changes in blood glucose levels depends on Insulin signaling as well as enzymes involved in the regulation of gluconeogenesis. Pathological changes to this process are central to the type 2 diabetes phenotype. Phosphoenolpyruvate carboxykinase (PEPCK) plays an important role in this process by stimulating hepatic glucose production. PEPCK expression increases in response to glucagon and glucocorticoids, while Insulin suppresses expression. Modulation of the signals governing PEPCK levels present a potential therapeutic approach to the treatment of Insulin resistance and consequently obesity. The cytosolic form of PEPCK, known as PEPCK-C, and the mitochondrial form, known as PEPCK-M, are encoded by two different nuclear genes in mouse, human and chicken.
Product Categories/Family for anti-PCK2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,564 Da
NCBI Official Full Name
phosphoenolpyruvate carboxykinase
NCBI Official Synonym Full Names
phosphoenolpyruvate carboxykinase 2 (mitochondrial)
NCBI Official Symbol
PCK2
NCBI Official Synonym Symbols
PEPCK; PEPCK2; PEPCK-M
NCBI Protein Information
phosphoenolpyruvate carboxykinase [GTP], mitochondrial; phosphoenolpyruvate carboxykinase [GTP], mitochondrial; PEP carboxykinase; phosphopyruvate carboxylase
UniProt Protein Name
Phosphoenolpyruvate carboxykinase [GTP], mitochondrial
Protein Family
UniProt Gene Name
PCK2
UniProt Synonym Gene Names
PEPCK2; PEPCK-M
UniProt Entry Name
PCKGM_HUMAN
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Product Notes
The PCK2 pck2 (Catalog #AAA9436551) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PCK2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PCK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF),Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:1000 IHC: 1:50-1:200 ICC: 1:50-1:100 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the PCK2 pck2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PCK2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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