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Western Blot (WB)
(Western blot analysis of Endo G on mouse heart tissue lysate using anti-Endo G antibody at 1/500 dilution.)
Rabbit anti-Human Endo G Monoclonal Antibody | anti-ENDOG antibody
Endo G Antibody
Reactivity
Human
Applications
Western Blot, Immunocytochemistry
Purity
ProA affinity purified
Synonyms
Endo G; Monoclonal Antibody; Endo G Antibody; EndoG antibody; Endonuclease G antibody; Endonuclease G mitochondrial antibody; EndonucleaseG antibody; FLJ27463 antibody; Mitochondrial endonuclease G antibody; NUCG_HUMAN antibody; anti-ENDOG antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
JU31-30
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-ENDOG antibody
Western Blot (WB), Immunocytochemistry (ICC)
Application Notes
WB: 1:500-1:1000
ICC: 1:50-1:200
ICC: 1:50-1:200
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western blot analysis of Endo G on mouse heart tissue lysate using anti-Endo G antibody at 1/500 dilution.)
Western Blot (WB)
(Western blot analysis of Endo G on mouse heart tissue lysate using anti-Endo G antibody at 1/500 dilution.)
Immunocytochemistry (ICC)
(ICC staining Endo G in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining Endo G in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining Endo G in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining Endo G in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining Endo G in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised)
Immunocytochemistry (ICC)
(ICC staining Endo G in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised)
Related Product Information for anti-ENDOG antibody
Endonuclease G (ENDOG), a nuclear encoded protein, localizes to the mitochondria. This sugar-nonspecific nuclease, responsible for major mitochondrial nuclease activity, preferentially cleaves single-stranded DNA (ssDNA). Synthesized as a propeptide with an amino-terminal presequence that targets the nuclease to mitochondria, ENDOG translocates to neuclei on apoptotic stimulation and act as a nuclease without sequence specificity. Both exonucleases and DNase I stimulate the ability of ENDOG to generate double-stranded DNA cleavage products at physiological ionic strengths, suggesting that these activities work in concert with ENDOG in apoptotic cells to ensure efficient DNA breakdown. In addition to deoxyribonuclease activities, ENDOG also has ribonuclease (RNase) and RNase H activities. ENDOG is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA. ENDOG exists in the mitochondrial intermembrane space, but not in the matrix where mtDNA replication occurs. This enzyme provides an important nicking function for mitochondrial DNA specifically cleaving DNA at GC tracts. Human ENDOG maps to chromosome 9q34.11.
Product Categories/Family for anti-ENDOG antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
297
NCBI Official Full Name
endonuclease G, mitochondrial
NCBI Official Synonym Full Names
endonuclease G
NCBI Official Symbol
ENDOG
NCBI Protein Information
endonuclease G, mitochondrial; endo G; mitochondrial endonuclease G
UniProt Protein Name
Endonuclease G, mitochondrial
Protein Family
UniProt Gene Name
ENDOG
UniProt Synonym Gene Names
Endo G
UniProt Entry Name
NUCG_HUMAN
Similar Products
Product Notes
The ENDOG endog (Catalog #AAA9436503) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Endo G Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Endo G can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC). WB: 1:500-1:1000 ICC: 1:50-1:200. Researchers should empirically determine the suitability of the ENDOG endog for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Endo G, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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