Western Blot (WB)
(Western blot analysis of MelanA on different lysates using anti-MelanA antibody at 1/1, 000 dilution. Positive control: Lane 1: Melanoma Lane 2: B16-F1)
Rabbit anti-Human MelanA Monoclonal Antibody | anti-MLANA antibody
MelanA Antibody
Gene Names
MLANA; MART1; MART-1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
MelanA; Monoclonal Antibody; MelanA Antibody; Antigen LB39 AA antibody; Antigen LB39-AA antibody; Antigen SK29 AA antibody; Antigen SK29-AA antibody; MAR1_HUMAN antibody; MART 1 antibody; MART-1 antibody; MART1 antibody; Melan A antibody; Melan A protein antibody; Melanoma antigen recognized by T cells 1 antibody; Melanoma antigen recognized by T-cells 1 antibody; MLAN A antibody; MLANA antibody; OTTHUMP00000021036 antibody; OTTHUMP00000021037 antibody; OTTHUMP00000021038 antibody; Protein Melan-A antibody; anti-MLANA antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
SC56-02
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-MLANA antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000-1:2000
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western blot analysis of MelanA on different lysates using anti-MelanA antibody at 1/1, 000 dilution. Positive control: Lane 1: Melanoma Lane 2: B16-F1)
Western Blot (WB)
(Western blot analysis of MelanA on different lysates using anti-MelanA antibody at 1/1, 000 dilution. Positive control: Lane 1: Melanoma Lane 2: B16-F1)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of B16-F1 cells with MelanA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the seconda antibody)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of B16-F1 cells with MelanA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the seconda antibody)
Related Product Information for anti-MLANA antibody
Melanoma-associated antigens recognized by cytotoxic T lymphocytes (CTL) have been grouped into three categories: melanocyte differentiation antigens, cancer/testis-specific antigens and mutated or aberrantly expressed antigens. Many of these antigens consist of peptides that are presented to T cells by HLA molecules; they represent potential targets for cancer immunotherapy. Melan-A (also designated MART-1) is a melanocyte differentiation antigen that is specific to melanomas, melanocyte cell lines and retina. Melan-A peptide is recognized by most HLA-A2-restricted tumor-specific tumor-infiltrating lymphocytes in patients with melanoma. Antimelanoma cytotoxic T lymphocytes can be generated with a Melan-A peptide, implicating Melan-A as a potential candidate for antigen-specific immunotherapy in melanoma patients.
Product Categories/Family for anti-MLANA antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13,157 Da
NCBI Official Full Name
melanoma antigen recognized by T-cells 1
NCBI Official Synonym Full Names
melan-A
NCBI Official Symbol
MLANA
NCBI Official Synonym Symbols
MART1; MART-1
NCBI Protein Information
melanoma antigen recognized by T-cells 1; antigen LB39-AA; antigen SK29-AA; protein Melan-A
UniProt Protein Name
Melanoma antigen recognized by T-cells 1
Protein Family
UniProt Gene Name
MLANA
UniProt Synonym Gene Names
MART1; MART-1
UniProt Entry Name
MAR1_HUMAN
Uniprot Description
MLANA: Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.
Protein type: Cell surface; Membrane protein, integral
Chromosomal Location of Human Ortholog: 9p24.1
Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; integral to plasma membrane; melanosome; trans-Golgi network
Molecular Function: protein binding
Research Articles on MLANA
Similar Products
Product Notes
The MLANA mlana (Catalog #AAA9435753) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MelanA Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MelanA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:1000-1:2000 IHC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the MLANA mlana for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MelanA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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