argonaute-2 Recombinant Protein | AGO2 recombinant protein

Recombinant Human Protein argonaute-2

Cat. Num. AAA9421473

• Gene Names: AGO2; PPD; Q10; CASC7; EIF2C2; LINC00980
• Reactivity: Human
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: argonaute-2; Recombinant Human Protein argonaute-2; Argonaute RISC catalytic component 2; AGO2 recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: 20mM Tris-HCl based buffer, pH8.0
• Sequence Positions: Piwi, 517-818aa

• Tag Info: His-tag
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C. Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

NCBI and Uniprot Product Information

• NCBI GI #: 257467482
• NCBI GeneID: 27161
• NCBI Accession #: NP_001158095.1
• NCBI GenBank Nucleotide #: NM_001164623.2
• UniProt Accession #: Q9UKV8; Q8TCZ5; Q8WV58; Q96ID1
• Molecular Weight: 38.1kD
• NCBI Official Full Name: protein argonaute-2 isoform 2
• NCBI Official Synonym Full Names: argonaute 2, RISC catalytic component
• NCBI Official Symbol: AGO2
• NCBI Official Synonym Symbols: PPD; Q10; CASC7; EIF2C2; LINC00980
• NCBI Protein Information: protein argonaute-2
• UniProt Protein Name: Protein argonaute-2
• Protein Family: Protein argonaute
• UniProt Gene Name: AGO2
• UniProt Synonym Gene Names: EIF2C2; Argonaute2; hAgo2; eIF-2C 2; eIF2C 2; PPD

NCBI Description

This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

AGO2: the endonuclease in RNA-induced silencing complexes (RISC) that cleaves siRNA/mRNA heteroduplexes bound to RISC. Ago2, along with Dicer and TRBP, are major components of RISC. Interacts with Dicer1 through its Piwi domain. Required for proper fibroblast growth factor signaling during gastrulation. Essential for embryonic development as well as RNA-mediated gene silencing (RNAi).

Protein type: EC 3.1.26.n2; RNA processing; RNA-binding

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: cell junction; cytoplasm; cytosol; membrane; mRNA cap complex; nucleoplasm; nucleus; polysome; ribonucleoprotein complex

Molecular Function: double-stranded RNA binding; endoribonuclease activity; miRNA binding; protein binding; protein C-terminus binding; RNA 7-methylguanosine cap binding; RNA binding; single-stranded RNA binding; siRNA binding

Biological Process: miRNA-mediated gene silencing, miRNA loading onto RISC; miRNA-mediated gene silencing, mRNA cleavage; miRNA-mediated gene silencing, negative regulation of translation; miRNA-mediated gene silencing, production of miRNAs; negative regulation of translational initiation; phosphoinositide-mediated signaling; positive regulation of transcription from RNA polymerase II promoter; pre-microRNA processing; RNA interference, production of siRNA; RNA interference, siRNA loading onto RISC; RNA secondary structure unwinding; RNA-mediated gene silencing; RNA-mediated posttranscriptional gene silencing; Wnt receptor signaling pathway, calcium modulating pathway