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Western Blot (WB) (Gel: 8%SDS-PAGE Lysate: 40 μ g Lane 1-3: Hela, hepg2 and HT-29 cell Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 5 seconds)

Rabbit anti-Human PTBP1 Polyclonal Antibody | anti-PTBP1 antibody

PTBP1 Antibody

Gene Names
PTBP1; PTB; PTB2; PTB3; PTB4; pPTB; HNRPI; PTB-1; PTB-T; HNRNPI; HNRNP-I
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purification
Synonyms
PTBP1; Polyclonal Antibody; PTBP1 Antibody; PTB; PTB2; PTB3; PTB4; pPTB; HNRPI; PTB-1; PTB-T; HNRNPI; HNRNP-I; anti-PTBP1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total PTBP1 protein.
Purity/Purification
Antigen Affinity Purification
Form/Format
Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
557
Applicable Applications for anti-PTBP1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot: 1:200-1:1000
Immunohistochemistry: 1:25-1:100
Immunogen
Fusion protein of human PTBP1
Immunogen Type
Protein
Preparation and Storage
Store at -20 degree C

Western Blot (WB)

(Gel: 8%SDS-PAGE Lysate: 40 μ g Lane 1-3: Hela, hepg2 and HT-29 cell Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 5 seconds)

Western Blot (WB) (Gel: 8%SDS-PAGE Lysate: 40 μ g Lane 1-3: Hela, hepg2 and HT-29 cell Primary antibody: 1/400 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 5 seconds)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue using at dilution 1/20.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Human prostate cancer tissue using at dilution 1/20.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using at dilution 1/20.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using at dilution 1/20.)
Related Product Information for anti-PTBP1 antibody
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm.
Product Categories/Family for anti-PTBP1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
59,633 Da
NCBI Official Full Name
polypyrimidine tract-binding protein 1 isoform a
NCBI Official Synonym Full Names
polypyrimidine tract binding protein 1
NCBI Official Symbol
PTBP1
NCBI Official Synonym Symbols
PTB; PTB2; PTB3; PTB4; pPTB; HNRPI; PTB-1; PTB-T; HNRNPI; HNRNP-I
NCBI Protein Information
polypyrimidine tract-binding protein 1
UniProt Protein Name
Polypyrimidine tract-binding protein 1
UniProt Gene Name
PTBP1
UniProt Synonym Gene Names
PTB; PTB; hnRNP I
UniProt Entry Name
PTBP1_HUMAN

Uniprot Description

hnRNP I: a ubiquitously expressed heterogeneous nuclear ribonucleoprotein (hnRNP). hnRNPs are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. Binds to the polypyrimidine tract of introns. May promote the binding of U2 snRNP to pre-mRNA. Two alternatively spliced isoforms have been described.

Protein type: RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: membrane; nucleolus; nucleoplasm

Molecular Function: poly-pyrimidine tract binding; protein binding; RNA binding

Biological Process: fibroblast growth factor receptor signaling pathway; gene expression; mRNA processing; negative regulation of muscle cell differentiation; negative regulation of nuclear mRNA splicing, via spliceosome; negative regulation of RNA splicing; nuclear mRNA splicing, via spliceosome; regulation of alternative nuclear mRNA splicing, via spliceosome; RNA splicing

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Product Notes

The PTBP1 ptbp1 (Catalog #AAA9416463) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PTBP1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PTBP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: 1:200-1:1000 Immunohistochemistry: 1:25-1:100. Researchers should empirically determine the suitability of the PTBP1 ptbp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PTBP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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