Western Blot (WB)
(Western blot detection of GST antibody in CHO-K1 transfected with GST cell lysate and GST recombinant protein fragments, purified recombinant human GST-Lin28B & GST-Lin28A protein fragments expressed in E Coli,using GST antibody (1:10000 diluted).)
Rabbit GST Polyclonal Antibody | anti-GST antibody
GST Polyclonal Antibody
Applications
Western Blot
Purity
Affinity purified
Synonyms
GST; Polyclonal Antibody; GST Polyclonal Antibody; Glutathione S Transferase; Glutathione S transferase Mu 1; Glutathione S-transferase class-mu 26 kDa isozyme; GST 26; GST class mu 1; GST1; GSTM1 1; GSTM1a 1a; GSTM1b 1b; GTH4; GTM1; H B; HB subunit 4; MGC26563; MU 1; MU; SJ26 antigen; SjGST.; anti-GST antibody
Host
Rabbit
Clonality
Polyclonal
Specificity
Transfected Only
Purity/Purification
Affinity purified
Form/Format
Purified Rabbit polyclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Applicable Applications for anti-GST antibody
Western Blot (WB)
Application Notes
Western blotting: 1:10000
Immunogen Type
Recombinant Protein
Immunogen Description
Purified recombinant Escherichia coli BL21(DE3) GST protein fragments expressed in E Coli.
Target Name
GST
Preparation and Storage
Store at -20 degree C
Western Blot (WB)
(Western blot detection of GST antibody in CHO-K1 transfected with GST cell lysate and GST recombinant protein fragments, purified recombinant human GST-Lin28B & GST-Lin28A protein fragments expressed in E Coli,using GST antibody (1:10000 diluted).)
Western Blot (WB)
(Western blot detection of GST antibody in CHO-K1 transfected with GST cell lysate and GST recombinant protein fragments, purified recombinant human GST-Lin28B & GST-Lin28A protein fragments expressed in E Coli,using GST antibody (1:10000 diluted).)
Related Product Information for anti-GST antibody
The glutathione S-transferase (GST, previously known as ligandins) family of enzymes are composed of many cytosolic, mitochondrial,and microsomal (now designated as MAPEG) proteins.GSTs are present in eukaryotes and in prokaryotes,where they catalyze a variety of reactions and accept endogenous and xenobiotic substrates.Members of the GST superfamily are extremely diverse in amino acid sequence,and a large fraction of the sequences deposited in public databases are of unknown function.The Enzyme Function Initiative (EFI) is using GSTs as a model superfamily to identify new GST functions.GSTs can constitute up to 10% of cytosolic protein in some mammalian organs.GSTs catalyse the conjugation of reduced glutathione — via a sulfhydryl group — to electrophilic centers on a wide variety of substrates in order to make the compounds more soluble.This activity detoxifies endogenous compounds such as peroxidised lipids, as well as breakdown of xenobiotics.GSTs may also bind toxins and function as transport proteins,which gave rise to the early term for GSTs of “ligandin”.The mammalian GST super-family consists of cytosolic dimeric isoenzymes of 45–55 kDa size that have been assigned to at least six classes:Alpha,Mu,Pi,Theta,Zeta and Omega.Most mammalian isoenzymes have affinity for the substrate 1-chloro-2,4-dinitrobenzene (CDNB),and spectrophotometric assays utilising this substrate are commonly used to report GST activity.However,some endogenous compounds,e.g., bilirubin,can inhibit the activity of GSTs.In mammals,GST isoforms have cell specific distributions (e.g., alpha GST in hepatocytes and pi GST in the biliary tract of the human liver).
Product Categories/Family for anti-GST antibody
Similar Products
Product Notes
The GST (Catalog #AAA9412847) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GST can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blotting: 1:10000. Researchers should empirically determine the suitability of the GST for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GST, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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