Western Blot (WB)
(Western Blot analysis of VEC cells using CHOP Polyclonal Antibody)
Rabbit anti-Human, Mouse CHOP Polyclonal Antibody | anti-CHOP antibody
CHOP Polyclonal Antibody
Immunohistochemistry: 1:100 - 1:300
Immunofluorescence: 1:200 - 1:1000
ELISA: 1:5000
Not yet tested in other applications.
Western Blot (WB)
(Western Blot analysis of VEC cells using CHOP Polyclonal Antibody)
Western Blot (WB)
(Western Blot analysis of VEC cells using CHOP Polyclonal Antibody)
Western Blot (WB)
(Western Blot analysis of HepG2 cells using CHOP Polyclonal Antibody)
Western Blot (WB)
(Western Blot analysis of HepG2 cells using CHOP Polyclonal Antibody)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010]
Uniprot Description
CHOP: a transcriptional-regulatory protein of the bZIP family. Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA. May play an important role in melanoma progression. CK2-mediated phosphorylation inhibits its transcriptional activity. Up-regulates IL-6 transcription by trapping negative regulating NF-IL6 isoform.
Protein type: Oncoprotein; Transcription factor; Autophagy; DNA-binding
Chromosomal Location of Human Ortholog: 12q13.1-q13.2
Cellular Component: nucleoplasm; late endosome; cytosol; nucleus
Molecular Function: leucine zipper domain binding; protein binding; DNA binding; protein heterodimerization activity; transcription factor binding; transcription corepressor activity; transcription factor activity
Biological Process: proteasomal ubiquitin-dependent protein catabolic process; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-8 production; regulation of transcription, DNA-dependent; positive regulation of neuron apoptosis; cell cycle arrest; ER overload response; regulation of transcription in response to stress; Wnt receptor signaling pathway; negative regulation of protein kinase B signaling cascade; unfolded protein response; negative regulation of transcription factor activity; mRNA transcription from RNA polymerase II promoter; blood vessel maturation; response to unfolded protein; response to starvation; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; cell redox homeostasis; release of sequestered calcium ion into cytosol; positive regulation of transcription from RNA polymerase II promoter; inhibition of CREB transcription factor; negative regulation of transcription, DNA-dependent; negative regulation of myoblast differentiation; response to DNA damage stimulus
Disease: Myxoid Liposarcoma
Research Articles on CHOP
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Product Notes
The CHOP ddit3 (Catalog #AAA9410117) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CHOP Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CHOP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:100 - 1:300 Immunofluorescence: 1:200 - 1:1000 ELISA: 1:5000 Not yet tested in other applications. Researchers should empirically determine the suitability of the CHOP ddit3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHOP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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