Rabbit anti-Human, Mouse TPM2 Polyclonal Antibody | anti-TPM2 antibody

TPM2 antibody

Cat. Num. AAA9409555

• Gene Names: TPM2; DA1; DA2B; NEM4; TMSB; AMCD1; HEL-S-273
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: TPM2; Polyclonal Antibody; TPM2 antibody; DA1; DA2B; NEM4; TMSB; AMCD1; anti-TPM2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total TPM2 antibody.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 284

• Applicable Applications for anti-TPM2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500 - 1:1000; Immunohistochemistry: 1:50 - 1:100
• Immunogen Type: Peptide
• Immunogen Description: A synthetic peptide of human TPM2.
• Target Name: TPM2
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using TPM2 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human lung cancer tissue using TPM2 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded rat testis tissue using TPM2 antibody at dilution of 1:200 (x400 lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human liver cancer tissue using TPM2 antibody at dilution of 1:200 (x400 lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human rectal cancer tissue using TPM2 antibody at dilution of 1:200 (x400 lens))

Related Product Information for anti-TPM2 antibody

This gene encodes beta-tropomyosin, a member of the actin filament binding protein family, and mainly expressed in slow, type 1 muscle fibers. Mutations in this gene can alter the expression of other sarcomeric tropomyosin proteins, and cause cap disease, nemaline myopathy and distal arthrogryposis syndromes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-TPM2 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 42476296
• NCBI GeneID: 7169
• NCBI Accession #: NP_003280.2
• NCBI GenBank Nucleotide #: NM_003289.3
• UniProt Accession #: P07951; P06468; Q13894; Q53FM4; Q5TCU4; Q5TCU7; Q9UH67; A6NM85
• Molecular Weight: SDS-PAGE MW: 33kd
• NCBI Official Full Name: tropomyosin beta chain isoform Tpm2.2st
• NCBI Official Synonym Full Names: tropomyosin 2 (beta)
• NCBI Official Symbol: TPM2
• NCBI Official Synonym Symbols: DA1; DA2B; NEM4; TMSB; AMCD1; HEL-S-273
• NCBI Protein Information: tropomyosin beta chain
• UniProt Protein Name: Tropomyosin beta chain
• Protein Family: Tropomyosin
• UniProt Gene Name: TPM2
• UniProt Synonym Gene Names: TMSB
• UniProt Entry Name: TPM2_HUMAN

NCBI Description

This gene encodes beta-tropomyosin, a member of the actin filament binding protein family, and mainly expressed in slow, type 1 muscle fibers. Mutations in this gene can alter the expression of other sarcomeric tropomyosin proteins, and cause cap disease, nemaline myopathy and distal arthrogryposis syndromes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009]

Uniprot Description

TPM2: Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. The non-muscle isoform may have a role in agonist-mediated receptor internalization. Defects in TPM2 are the cause of nemaline myopathy type 4 (NEM4). A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. Nemaline myopathy type 4 presents from infancy to childhood with hypotonia and moderate-to-severe proximal weakness with minimal or no progression. Major motor milestones are delayed but independent ambulation is usually achieved, although a wheelchair may be needed in later life. Defects in TPM2 are the cause of distal arthrogryposis type 1A (DA1A). A form of distal arthrogryposis, a disease characterized by congenital joint flexures or contractures that mainly involve the distal parts of the limbs, and affect two or more different body areas in the absence of a primary neurological or muscle disease. Distal arthrogryposis type 1 is characterized largely by camptodactyly and clubfoot. Hypoplasia and/or absence of some interphalangeal creases is common. The shoulders and hips are less frequently affected. Belongs to the tropomyosin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 9p13

Cellular Component: cytosol; muscle thin filament tropomyosin

Molecular Function: structural constituent of muscle; actin binding

Biological Process: regulation of ATPase activity; muscle contraction; muscle filament sliding

Disease: Nemaline Myopathy 4; Arthrogryposis, Distal, Type 1a; Myopathy, Congenital, With Fiber-type Disproportion; Arthrogryposis, Distal, Type 2b