Rabbit DHFR Polyclonal Antibody | anti-DHFR antibody

DHFR Antibody

Cat. Num. AAA9407098

• Gene Names: DHFR; DYR; DHFRP1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: DHFR; Polyclonal Antibody; DHFR Antibody; DYR; DHFRP1; anti-DHFR antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total DHFR protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 187

• Applicable Applications for anti-DHFR antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:100
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human DHFR.
• Target Name: DHFR
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using DHFR antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat kidney using DHFR antibody at dilution of 1:100 (200x lens).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse lung using DHFR antibody at dilution of 1:100 (200x lens).)

Related Product Information for anti-DHFR antibody

Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia.

Product Categories/Family for anti-DHFR antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4503323
• NCBI GeneID: 1719
• NCBI Accession #: NP_000782.1
• NCBI GenBank Nucleotide #: NM_000791.3
• UniProt Accession #: P00374; Q14130; Q6IRW8; B4DDD2
• Molecular Weight: 15,672 Da
• NCBI Official Full Name: dihydrofolate reductase isoform 1
• NCBI Official Synonym Full Names: dihydrofolate reductase
• NCBI Official Symbol: DHFR
• NCBI Official Synonym Symbols: DYR; DHFRP1
• NCBI Protein Information: dihydrofolate reductase
• UniProt Protein Name: Dihydrofolate reductase
• Protein Family: Viral dihydrofolate reductase
• UniProt Gene Name: DHFR
• UniProt Entry Name: DYR_HUMAN

NCBI Description

Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014]

Uniprot Description

Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1.