Rabbit anti-Human, Mouse E2F2 Polyclonal Antibody | anti-E2F2 antibody

E2F2 Antibody

Cat. Num. AAA9403767

• Gene Names: E2F2; E2F-2
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: E2F2; Polyclonal Antibody; E2F2 Antibody; Transcription factor E2F2; E2F-2; anti-E2F2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total E2F2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 437

• Applicable Applications for anti-E2F2 antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500~1:3000
• Immunogen Type: Peptide
• Immunogen Description: Synthesized peptide derived from internal of human E2F2.
• Target Name: E2F2
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts from HepG2 cells, K562 cells and COLO205 cells, using E2F2 antibody.)

Related Product Information for anti-E2F2 antibody

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F2 binds specifically to RB1 in a cell-cycle dependent manner.Ivey-Hoyle M., Mol. Cell. Biol. 13:7802-7812(1993).Gregory S.G., Nature 441:315-321(2006).Novy M., Mol. Biol. Cell 16:2181-2190(2005).

Product Categories/Family for anti-E2F2 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4758226
• NCBI GeneID: 1870
• NCBI Accession #: NP_004082.1
• NCBI GenBank Nucleotide #: NM_004091.3
• UniProt Accession #: Q14209; Q7Z6H1; B2R9W1
• Molecular Weight: 47,506 Da
• NCBI Official Full Name: transcription factor E2F2
• NCBI Official Synonym Full Names: E2F transcription factor 2
• NCBI Official Symbol: E2F2
• NCBI Official Synonym Symbols: E2F-2
• NCBI Protein Information: transcription factor E2F2
• UniProt Protein Name: Transcription factor E2F2
• Protein Family: Transcription factor
• UniProt Gene Name: E2F2
• UniProt Synonym Gene Names: E2F-2
• UniProt Entry Name: E2F2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46% amino acid identity to E2F1. [provided by RefSeq, Jul 2008]

Uniprot Description

E2F2: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F2 binds specifically to RB1 in a cell-cycle dependent manner. Belongs to the E2F/DP family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: nucleoplasm; transcription factor complex

Molecular Function: protein binding; DNA binding; transcription factor binding; transcription factor activity

Biological Process: transcription initiation from RNA polymerase II promoter; regulation of transcription, DNA-dependent; regulation of cell cycle; mitotic cell cycle