Rabbit anti-Human DAP3 Polyclonal Antibody | anti-DAP3 antibody

DAP3 Antibody

Cat. Num. AAA9402919

• Gene Names: DAP3; DAP-3; MRPS29; MRP-S29; bMRP-10
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Synonyms: DAP3; Polyclonal Antibody; DAP3 Antibody; death associated protein 3; DAP-3; MRPS29; MRP-S29; bMRP-10; anti-DAP3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total DAP3 protein.
• Form/Format: Supplied at 0.6mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.
• Sequence Length: 1474

• Applicable Applications for anti-DAP3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: ELISA: 1:1000-1:5000; Western blotting: 1:200-1:1000; Immunohistochemistry: 1:5-1:20
• Immunogen Type: Recombinant protein
• Immunogen Description: Fusion protein corresponding to a C terminal 300 amino acids of human death associated protein 3
• Target Name: DAP3
• Preparation and Storage: Store at -20 degree C/1 year

Testing Data

(Gel: 12%SDS-PAGELane1: 293T cell lysateLane2: Jurkat cell lysateLane3: Human Lymphoma tissue lysateLysates: 30ug per lanePrimary antibody: 1/300 dilutionSecondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilutionExposure time: 20 seconds)

Immunohistochemistry (IHC)

(The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 31066 (DAP3 Antibody) at dilution 1/10, on the right is treated with the fusion protein.)

Related Product Information for anti-DAP3 antibody

This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q.

Product Categories/Family for anti-DAP3 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 77567641
• NCBI GeneID: 7818
• Molecular Weight: 41,673 Da
• NCBI Official Full Name: Homo sapiens death associated protein 3, mRNA
• NCBI Official Synonym Full Names: death associated protein 3
• NCBI Official Symbol: DAP3
• NCBI Official Synonym Symbols: DAP-3; MRPS29; MRP-S29; bMRP-10
• NCBI Protein Information: 28S ribosomal protein S29, mitochondrial
• UniProt Protein Name: 28S ribosomal protein S29, mitochondrial
• Protein Family: 28S ribosomal protein
• UniProt Gene Name: DAP3
• UniProt Synonym Gene Names: MRPS29; MRP-S29; S29mt; DAP-3
• UniProt Entry Name: RT29_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. [provided by RefSeq, Dec 2010]

Uniprot Description

DAP3: Involved in mediating interferon-gamma-induced cell death. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Nuclear receptor co-regulator; Apoptosis

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: nucleoplasm; small ribosomal subunit; mitochondrion; mitochondrial inner membrane; nucleolus; mitochondrial ribosome; nucleus

Molecular Function: protein binding

Biological Process: apoptotic mitochondrial changes; mitochondrial translation; organelle organization and biogenesis