Western Blot (WB)
(Western Blot analysis of COLO cells using Phospho-Net (S357) Polyclonal Antibody)
Rabbit Net Polyclonal Antibody | anti-Net antibody
Net (Phospho-Ser357) Polyclonal Antibody
Immunohistochemistry: 1:100 - 1:300
Immunofluorescence: 1:200 - 1:1000
ELISA: 1:20000
Not yet tested in other applications
Western Blot (WB)
(Western Blot analysis of COLO cells using Phospho-Net (S357) Polyclonal Antibody)
Western Blot (WB)
(Western Blot analysis of COLO cells using Phospho-Net (S357) Polyclonal Antibody)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the ETS-domain transcription factor family and the ternary complex factor (TCF) subfamily. Proteins in this subfamily regulate transcription when recruited by serum response factor to bind to serum response elements. This protein is activated by signal-induced phosphorylation; studies in rodents suggest that it is a transcriptional inhibitor in the absence of Ras, but activates transcription when Ras is present. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]
Uniprot Description
ELK3: a transcription factor of the ETS-domain transcription factor family and the ternary complex factor (TCF) subfamily. May be a negative regulator of transcription but can activate transcription when co expressed with Ras, Src or Mos. Activated by signal-induced phosphorylation; studies in rodents suggest that it is a transcriptional inhibitor in the absence of Ras, but activates transcription when Ras is present.
Protein type: Transcription factor; DNA-binding
Chromosomal Location of Human Ortholog: 12q23
Cellular Component: nucleoplasm; mitochondrion; intracellular membrane-bound organelle; nucleus
Molecular Function: protein binding; transcription corepressor activity; transcription factor activity; purine-rich negative regulatory element binding
Biological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; wound healing; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; negative regulation of transcription, DNA-dependent; cell differentiation; signal transduction
Research Articles on Net
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Product Notes
The Net elk3 (Catalog #AAA9402348) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Net (Phospho-Ser357) Polyclonal Antibody reacts with Human, Mouse, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's Net can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). Western blotting: 1:500 - 1:2000 Immunohistochemistry: 1:100 - 1:300 Immunofluorescence: 1:200 - 1:1000 ELISA: 1:20000 Not yet tested in other applications. Researchers should empirically determine the suitability of the Net elk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Net, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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