Rabbit MAPKAPK-2 Polyclonal Antibody | anti-MAPKAPK-2 antibody

MAPKAPK-2 (Phospho-Thr334) Antibody

Cat. Num. AAA9401293

• Gene Names: MAPKAPK2; MK2; MK-2; MAPKAP-K2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: MAPKAPK-2; Polyclonal Antibody; MAPKAPK-2 (Phospho-Thr334) Antibody; MAP kinase-activated protein kinase 2; MAPK-activated protein kinase 2; MAPK2; MAPKAP kinase 2; anti-MAPKAPK-2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 370

• Applicable Applications for anti-MAPKAPK-2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500~1:1000; Immunohistochemistry: 1:50~1:100; Immunofluorescence: 1:100~1:200
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of threonine 334 (P-Q-T(p)-P-L) derived from Human MAPKAPK-2.
• Modification: Phospho-Thr334
• Target Name: MAPKAPK-2
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Western Blot (WB)

(Western blot analysis of extracts from Hela cells untreated(lane 1) or treated with UV(lane 2) using MAPKAPK-2(Phospho-Thr334) Antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MAPKAPK-2(Phospho-Thr334) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

Immunofluorescence (IF)

(Immunofluorescence staining of methanol-fixed Hela cells using MAPKAPK-2(Phospho-Thr334) Antibody.)

Related Product Information for anti-MAPKAPK-2 antibody

MAPKAPK-2 encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.

Product Categories/Family for anti-MAPKAPK-2 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 10863901
• NCBI GeneID: 9261
• NCBI Accession #: NP_004750.1
• NCBI GenBank Nucleotide #: NM_004759.4
• UniProt Accession #: P49137; Q5SY30; Q5SY41; Q8IYD6
• Molecular Weight: 42,203 Da
• NCBI Official Full Name: MAP kinase-activated protein kinase 2 isoform 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase-activated protein kinase 2
• NCBI Official Symbol: MAPKAPK2
• NCBI Official Synonym Symbols: MK2; MK-2; MAPKAP-K2
• NCBI Protein Information: MAP kinase-activated protein kinase 2
• UniProt Protein Name: MAP kinase-activated protein kinase 2
• UniProt Gene Name: MAPKAPK2
• UniProt Synonym Gene Names: MAPK-activated protein kinase 2; MAPKAP kinase 2; MAPKAP-K2; MAPKAPK-2; MK-2; MK2
• UniProt Entry Name: MAPK2_HUMAN

NCBI Description

This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

MAPKAPK2: a member of the MAPKAPK family of protein kinases. It is phosphorylated and activated by p38 in response to cytokines, stress and chemotactic factors.MAPKAPK-2 is apparently a direct target of p38 MAPK. HSP27 is one of its in vivo substrates. Two transcript variants encoding two different isoforms have been described.

Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, CAMK; CAMK group; MAPKAPK family; MAPKAPK subfamily

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: nucleoplasm; centrosome; cytoplasm; nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; signal transducer activity; ATP binding; protein kinase activity

Biological Process: nerve growth factor receptor signaling pathway; activation of MAPK activity; stress-activated MAPK cascade; leukotriene metabolic process; response to lipopolysaccharide; toll-like receptor 3 signaling pathway; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; regulation of interleukin-6 production; response to stress; arachidonic acid metabolic process; inflammatory response; inner ear development; toll-like receptor 4 signaling pathway; MyD88-independent toll-like receptor signaling pathway; MAPKKK cascade; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; peptidyl-serine phosphorylation; response to cytokine stimulus; Ras protein signal transduction; toll-like receptor signaling pathway; innate immune response; gene expression; regulation of tumor necrosis factor production; toll-like receptor 9 signaling pathway; G2/M transition DNA damage checkpoint; vascular endothelial growth factor receptor signaling pathway; response to DNA damage stimulus