Rabbit KRT20 Polyclonal Antibody | anti-KRT20 antibody

KRT20 Antibody

Cat. Num. AAA9400927

• Gene Names: KRT20; K20; CD20; CK20; CK-20; KRT21
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: KRT20; Polyclonal Antibody; KRT20 Antibody; K20; Cytokeratin20; KRT21; CK20; anti-KRT20 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total KRT20 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 424

• Applicable Applications for anti-KRT20 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:200; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human KRT20.
• SDS-PAGE MW: 48kD
• Target Name: KRT20
• Preparation and Storage: Store at -20°C

Western Blot (WB)

(Western blot analysis of extracts from Hela cell and mouse intestines tissue, using KRT20 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human colon cancer using KRT20 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human rectal cancer using KRT20 antibody at dilution of 1:200 (200x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using KRT20 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-KRT20 antibody

Intermediate-sized filament (IF) protein designated cytokeratin (CK) 20 is a major cellular protein of mature enterocytes and goblet cells commonly found in mucosal epithelium of the mammalian gastrointestinal tract (1). Results strongly suggest that transcriptional regulation of keratin genes in the intestinal epithelium occurs at the level of both immature and terminally differentiated epithelial cells, and is tightly regulated during both fetal development and crypt-to-villus differentiation of the intestinal epithelium (2). CK20 has recently been reported to be useful to distinguish between primary and metastatic lung adenocarcinoma. CK20 expression was significantly more prevalent in adenocarcinoma that originated in the GI tract than that of pulmonary or breast origin (3).

Product Categories/Family for anti-KRT20 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 27894337
• NCBI GeneID: 54474
• NCBI Accession #: NP_061883.1
• NCBI GenBank Nucleotide #: NM_019010.2
• UniProt Accession #: P35900; B2R6W7
• NCBI Official Full Name: keratin, type I cytoskeletal 20
• NCBI Official Synonym Full Names: keratin 20, type I
• NCBI Official Symbol: KRT20
• NCBI Official Synonym Symbols: K20; CD20; CK20; CK-20; KRT21
• NCBI Protein Information: keratin, type I cytoskeletal 20
• UniProt Protein Name: Keratin, type I cytoskeletal 20
• Protein Family: Keratin
• UniProt Gene Name: KRT20
• UniProt Synonym Gene Names: CK-20; K20
• UniProt Entry Name: K1C20_HUMAN

NCBI Description

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]

Uniprot Description

K20: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: cytoplasm; intermediate filament

Molecular Function: protein binding; structural constituent of cytoskeleton

Biological Process: regulation of protein secretion; apoptosis; cellular response to stress; intermediate filament organization