Mouse CDC2 Monoclonal Antibody | anti-CDC2 antibody

CDC2 Monoclonal Antibody

Cat. Num. AAA9400324

• Gene Names: CDK1; CDC2; CDC28A; P34CDC2
• Reactivity: Human, Dog, Rat, Mouse
• Applications: Western Blot, Immunocytochemistry
• Purity: Purified by antigen-affinity chromatography.
• Synonyms: CDC2; Monoclonal Antibody; CDC2 Monoclonal Antibody; cyclin-dependent kinase 1 p34 protein kinase cell cycle controller CDC2 cell division protein kinase 1 cell division control protein 2 homolog cell division cycle 2; G1 to S and G2 to M; anti-CDC2 antibody

• Host: Mouse
• Reactivity: Human, Dog, Rat, Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Purity/Purification: Purified by antigen-affinity chromatography.
• Form/Format: Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
• Sequence Length: 297

• Applicable Applications for anti-CDC2 antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: Western blotting: 1:1000
• Immunogen Type: Recombinant protein
• Immunogen Description: Recombinant fragment, corresponding to amino acids 8-298aa of human CDC2.
• Target Name: CDC2
• Preparation and Storage: store at -20 degree C

Testing Data

(1:1000 dilution of this antibody detected CDC2 on 40ug of cell lysates)

Testing Data

(HeLa cells using anti- CDC2 antibody diluted 1:50)

Product Categories/Family for anti-CDC2 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4502709
• NCBI GeneID: 983
• NCBI Accession #: NP_001777.1
• NCBI GenBank Nucleotide #: NM_001786.4
• UniProt Accession #: P06493; O60764; A8K7C4; C9J497
• Molecular Weight: 27,503 Da
• NCBI Official Full Name: cyclin-dependent kinase 1 isoform 1
• NCBI Official Synonym Full Names: cyclin-dependent kinase 1
• NCBI Official Symbol: CDK1
• NCBI Official Synonym Symbols: CDC2; CDC28A; P34CDC2
• NCBI Protein Information: cyclin-dependent kinase 1
• UniProt Protein Name: Cyclin-dependent kinase 1
• Protein Family: CDC25-like phosphatase
• UniProt Gene Name: CDK1
• UniProt Synonym Gene Names: CDC2; CDC28A; CDKN1; P34CDC2; CDK1
• UniProt Entry Name: CDK1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Uniprot Description

CDK1: a protein kinase of the CDK family. Catalytic subunit of the conserved protein complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions. Mitotic cyclins stably associate with this protein and function as regulatory subunits. Its activity is controlled by cyclin availability and phosphorylation through the cell cycle. Activated in many cancers including colon, liver and breast. The T isoform, which lacks a regulatory region, is expressed in breast cancer. Inhibition in cancer cells may drive cells into apoptosis. May also drive cell migration. Inhibitors: BMS-265246, BMS-265246-01. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cell cycle regulation; Protein kinase, CMGC; EC 2.7.11.23; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.22; CMGC group; CDK family; CDK1 subfamily; CDK/CDK1 subfamily

Chromosomal Location of Human Ortholog: 10q21.1

Cellular Component: centrosome; cytoplasm; cytosol; membrane; midbody; mitochondrion; nuclear chromosome, telomeric region; nucleoplasm; nucleus; spindle microtubule

Molecular Function: ATP binding; cyclin binding; cyclin-dependent protein kinase activity; histone kinase activity; Hsp70 protein binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; RNA polymerase subunit kinase activity

Biological Process: activation of MAPK activity; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; apoptosis; cell aging; cell division; cell migration; centrosome cycle; chromosome condensation; DNA repair; DNA replication; epithelial cell differentiation; G1/S transition of mitotic cell cycle; G1/S-specific transcription in mitotic cell cycle; G2/M transition of mitotic cell cycle; histone phosphorylation; microtubule cytoskeleton organization and biogenesis; mitosis; mitotic cell cycle; mitotic cell cycle G2/M transition DNA damage checkpoint; mitotic nuclear envelope disassembly; negative regulation of apoptosis; organ regeneration; organelle organization and biogenesis; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; positive regulation of cardiac muscle cell proliferation; positive regulation of DNA replication; positive regulation of mitotic cell cycle; positive regulation of protein import into nucleus, translocation; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; pronuclear fusion; protein complex assembly; regulation of embryonic development; regulation of Schwann cell differentiation; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; response to activity; response to amine stimulus; response to axon injury; response to cadmium ion; response to copper ion; response to drug; response to ethanol; response to organic cyclic substance; response to toxin; ventricular cardiac muscle cell development