Typical Testing Data/Standard Curve (for reference only)
Mouse carnitine palmitoyltransferase 1A (liver) ELISA Kit | CPT1A elisa kit
Mouse Carnitine O-palmitoyltransferase 1, liver isoform, CPT1A ELISA Kit
Gene Names
Cpt1a; CPTI; Cpt1; C730027G07
Reactivity
Mouse
Synonyms
carnitine palmitoyltransferase 1A (liver); Mouse Carnitine O-palmitoyltransferase 1; liver isoform; CPT1A ELISA Kit; liver isoform (CPT1A) ELISA kit; CPT1; CPT1-L; L-CPT1; carnitine palmitoyltransferase 1A liver; carnitine palmitoyltransferase I; liver; CPT1A elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse CPT1A. No significant cross-reactivity or interference between mouse CPT1A and analogues was observed.
Sequence Length
773
Samples
Serum, plasma, Cell lysates, tissue homogenates
Assay Type
Sandwich
Detection Range
6.25 pg/ml -400 pg/ml.
Sensitivity
The minimum detectable dose of mouse CPT1A is typically less than 1.56 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CPT1A elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CPT1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CPT1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CPT1A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CPT1A bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
88,251 Da
NCBI Official Full Name
carnitine O-palmitoyltransferase 1, liver isoform
NCBI Official Synonym Full Names
carnitine palmitoyltransferase 1a, liver
NCBI Official Symbol
Cpt1a
NCBI Official Synonym Symbols
CPTI; Cpt1; C730027G07
NCBI Protein Information
carnitine O-palmitoyltransferase 1, liver isoform; CPT I; CPT1-L; CPTI-L; L-CPT I; carnitine palmitoyltransferase 1, liver; carnitine O-palmitoyltransferase I, liver isoform
UniProt Protein Name
Carnitine O-palmitoyltransferase 1, liver isoform
Protein Family
UniProt Gene Name
Cpt1a
UniProt Synonym Gene Names
Cpt-1; Cpt1; CPT1-L; CPT I; CPTI-L
UniProt Entry Name
CPT1A_MOUSE
Uniprot Description
CPT1A: Belongs to the carnitine/choline acetyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.3.1.21; Membrane protein, integral; Transferase; Lipid Metabolism - fatty acid; Mitochondrial; Membrane protein, multi-pass
Cellular Component: mitochondrial outer membrane; mitochondrion; intracellular membrane-bound organelle; membrane; mitochondrial inner membrane; integral to membrane; integral to mitochondrial outer membrane
Molecular Function: transferase activity; identical protein binding; transferase activity, transferring acyl groups; carnitine O-palmitoyltransferase activity
Biological Process: triacylglycerol metabolic process; eating behavior; transport; regulation of fatty acid oxidation; glucose metabolic process; carnitine metabolic process; lipid metabolic process; long-chain fatty acid metabolic process; fatty acid metabolic process; regulation of insulin secretion; protein homooligomerization; positive regulation of fatty acid beta-oxidation
Research Articles on CPT1A
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Product Notes
The Mouse CPT1A cpt1a (Catalog #AAA937269) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA937269 ELISA Kit recognizes Mouse CPT1A. It is sometimes possible for the material contained within the vial of "carnitine palmitoyltransferase 1A (liver), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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