Mouse sorbin and SH3 domain containing 1 ELISA Kit | SORBS1 elisa kit

Mouse Sorbin and SH3 domain-containing protein 1, SORBS1 ELISA Kit

Cat. Num. AAA9334603

• Gene Names: Sorbs1; CAP; Sh3d5; SH3P12; mKIAA1296; 2310065E01Rik; 9530001P15Rik
• Reactivity: Mouse
• Synonyms: sorbin and SH3 domain containing 1; Mouse Sorbin and SH3 domain-containing protein 1; SORBS1 ELISA Kit; Mouse Sorbin and SH3 domain-containing protein 1 (SORBS1) ELISA kit; CAP; DKFZp451C066; DKFZp586P1422; FLAF2; FLJ12406; KIAA1296; R85FL; SH3D5; SH3P12; SORB1; Fas-ligand associated factor 2; OTTHUMP00000020142; OTTHUMP00000020143; OTTHUMP00000020144; OTTHUMP00000020145; S; SORBS1 elisa kit

• Reactivity: Mouse
• Specificity: No significant cross-reactivity or interference between Mouse SORBS1 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 31.2pg/ml-1000pg/ml.
• Sensitivity: 5.0pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated SORBS1 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse SORBS1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SORBS1 and SORBS1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SORBS1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for SORBS1 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated SORBS1 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse SORBS1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SORBS1 and SORBS1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SORBS1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 78000179
• NCBI GeneID: 20411
• NCBI Accession #: NP_001030134.1
• NCBI GenBank Nucleotide #: NM_001034962.1
• UniProt Accession #: Q62417; Q80TF8; Q8BZI3; Q8K3Y2; Q921F8; Q9Z0Z8; Q9Z0Z9
• Molecular Weight: 143,070 Da
• NCBI Official Full Name: sorbin and SH3 domain-containing protein 1 isoform 3
• NCBI Official Synonym Full Names: sorbin and SH3 domain containing 1
• NCBI Official Symbol: Sorbs1
• NCBI Official Synonym Symbols: CAP; Sh3d5; SH3P12; mKIAA1296; 2310065E01Rik; 9530001P15Rik
• NCBI Protein Information: sorbin and SH3 domain-containing protein 1; ponsin; SH3 domain protein 5; c-Cbl-associated protein
• UniProt Protein Name: Sorbin and SH3 domain-containing protein 1
• Protein Family: Sorbin and SH3 domain-containing protein
• UniProt Gene Name: Sorbs1
• UniProt Synonym Gene Names: Kiaa1296; Sh3d5; CAP
• UniProt Entry Name: SRBS1_MOUSE

Uniprot Description

SORBS1: Plays a role in tyrosine phosphorylation of CBL by linking CBL to the insulin receptor. Required for insulin- stimulated glucose transport. Involved in formation of actin stress fibers and focal adhesions. 12 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Cytoskeletal

Cellular Component: centrosome; nuclear matrix; focal adhesion; flotillin complex; cytosol; cell-substrate adherens junction; lipid raft; nucleoplasm; cytoskeleton; cell-cell adherens junction; membrane; cytoplasm; plasma membrane; stress fiber; cell junction; nucleus

Molecular Function: protein binding; SH3/SH2 adaptor activity; transcription factor binding; protein kinase binding; insulin receptor binding

Biological Process: focal adhesion formation; positive regulation of glucose import; positive regulation of signal transduction; cellular response to insulin stimulus; transport; positive regulation of glycogen biosynthetic process; insulin receptor signaling pathway; stress fiber formation; actin filament organization; positive regulation of lipid biosynthetic process; glucose transport