Mouse piwi-like 2 (Drosophila) ELISA Kit | PIWIL2 elisa kit

Mouse Piwi-like protein 2, PIWIL2 ELISA Kit

Cat. Num. AAA9334308

• Gene Names: Piwil2; mili; Piwil1l
• Reactivity: Mouse
• Synonyms: piwi-like 2 (Drosophila); Mouse Piwi-like protein 2; PIWIL2 ELISA Kit; Mouse Piwi-like protein 2 (PIWIL2) ELISA kit; CT80; FLJ10351; HILI; MGC133049; PIWIL1L; mili; Hiwi-like; Miwi like; cancer/testis antigen 80; piwi-like 2; PIWIL2 elisa kit

• Reactivity: Mouse
• Specificity: No significant cross-reactivity or interference between Human PIWIL2 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 3.12ng/ml-100ng/ml.
• Sensitivity: 1.0ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated PIWIL2 concentrations in Human serum, plasma and other body fluids. Using Purified Human PIWIL2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PIWIL2 and PIWIL2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PIWIL2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for PIWIL2 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated PIWIL2 concentrations in Human serum, plasma and other body fluids. Using Purified Human PIWIL2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PIWIL2 and PIWIL2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PIWIL2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 10946610
• NCBI GeneID: 57746
• NCBI Accession #: NP_067283.1
• NCBI GenBank Nucleotide #: NM_021308.1
• UniProt Accession #: Q8CDG1; Q3TQE8; Q99MV6; Q9JMB6; A6H617
• Molecular Weight: 109,488 Da
• NCBI Official Full Name: piwi-like protein 2
• NCBI Official Synonym Full Names: piwi-like RNA-mediated gene silencing 2
• NCBI Official Symbol: Piwil2
• NCBI Official Synonym Symbols: mili; Piwil1l
• NCBI Protein Information: piwi-like protein 2; Miwi like; Miwi likw; piwi like homolog 2; piwi-like homolog 2
• UniProt Protein Name: Piwi-like protein 2
• Protein Family: Piwi-like protein
• UniProt Gene Name: Piwil2
• UniProt Synonym Gene Names: Mili
• UniProt Entry Name: PIWL2_MOUSE

Uniprot Description

PIWIL2: Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate in similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piRNA process acts upstream of known mediators of DNA methylation. Participates in a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. Inhibits tumor cell growth when repressed. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors. Interacts with DDX4, MAEL, EIF3A, EIF4E, EIF4G, PRMT5 and WDR77. Associates with EIF4E- and EIF4G-containing m7G cap-binding complexes. Interacts (when methylated on arginine residues) with TDRD1. Expressed in adult testis and in most tumors. Belongs to the argonaute family. Piwi subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cancer Testis Antigen (CTA); Apoptosis

Cellular Component: polysome; cytoplasm

Molecular Function: mRNA binding; protein binding; nucleic acid binding; RNA binding

Biological Process: regulation of translation; oogenesis; meiotic cell cycle; positive regulation of translation; multicellular organismal development; DNA methylation during gametogenesis; spermatogenesis; germ-line stem cell maintenance; RNA-mediated gene silencing; cell differentiation