Human ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) ELISA Kit | UCHL3 elisa kit

Human Ubiquitin carboxyl-terminal hydrolase isozyme L3, UCHL3 ELISA Kit

Cat. Num. AAA9333757

• Gene Names: UCHL3; UCH-L3
• Reactivity: Human
• Synonyms: ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase); Human Ubiquitin carboxyl-terminal hydrolase isozyme L3; UCHL3 ELISA Kit; Human Ubiquitin carboxyl-terminal hydrolase isozyme L3 (UCHL3) ELISA kit; RP11-173B14.3; UCH-L3; ubiquitin carboxyl-terminal esterase L3; ubiquitin thiolesterase; UCHL3 elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human UCHL3 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 0.5ng/ml-16ng/ml.
• Sensitivity: 0.1ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated UCHL3 concentrations in Human serum, plasma and other body fluids. Using Purified Human UCHL3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add UCHL3 and UCHL3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of UCHL3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for UCHL3 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated UCHL3 concentrations in Human serum, plasma and other body fluids. Using Purified Human UCHL3 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add UCHL3 and UCHL3 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of UCHL3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 401709950
• NCBI GeneID: 7347
• NCBI Accession #: NP_001257881.1
• NCBI GenBank Nucleotide #: NM_001270952.1
• UniProt Accession #: P15374; Q5TBK8; Q6IBE9; B2R970
• Molecular Weight: 26,183 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase isozyme L3 isoform 1
• NCBI Official Synonym Full Names: ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
• NCBI Official Symbol: UCHL3
• NCBI Official Synonym Symbols: UCH-L3
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase isozyme L3; ubiquitin thiolesterase; ubiquitin thioesterase L3
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase isozyme L3
• Protein Family: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: UCHL3
• UniProt Synonym Gene Names: UCH-L3
• UniProt Entry Name: UCHL3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the deubiquitinating enzyme family. Members of this family are proteases that catalyze the removal of ubiquitin from polypeptides and are divided into five classes, depending on the mechanism of catalysis. This protein may hydrolyze the ubiquitinyl-N-epsilon amide bond of ubiquitinated proteins to regenerate ubiquitin for another catalytic cycle. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Aug 2012]

Uniprot Description

UCHL3: Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin- signaling and insulin-induced adipogenesis. Required for stress- response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders. Preferentially binds diubiquitin; the interaction does not hydrolyze diubiquitin but, in vitro, inhibits the hydrolyzing activity on other substrates. Highly expressed in heart, skeletal muscle, and testis. Inhibited by monoubiquitin and diubiquitin. Belongs to the peptidase C12 family.

Protein type: Protease; Ubiquitin conjugating system; EC 3.4.19.12

Chromosomal Location of Human Ortholog: 13q22.2

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: peptidase activity; ubiquitin binding; protein binding; ubiquitin-specific protease activity

Biological Process: ubiquitin-dependent protein catabolic process; protein catabolic process