Rabbit protein phosphatase 4 (formerly X) , catalytic subunit ELISA Kit | PPP4C elisa kit

Rabbit Serine/threonine-protein phosphatase 4 Catalytic subunit, PPP4C ELISA Kit

• Gene Names: PPP4C; PPX
• Reactivity: Rabbit
• Synonyms: protein phosphatase 4 (formerly X); catalytic subunit; Rabbit Serine/threonine-protein phosphatase 4 Catalytic subunit; PPP4C ELISA Kit; Rabbit Serine/threonine-protein phosphatase 4 catalytic subunit (PPP4C) ELISA kit; PP4; PP4C; PPH3; PPP4; PPX; protein phosphatase 4; protein phosphatase X; serine/threonine-protein phosphatase 4; PPP4C elisa kit

• Reactivity: Rabbit
• Specificity: No significant cross-reactivity or interference between Rabbit PPP4C and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 31.2pg/ml-1000pg/ml.
• Sensitivity: 5.0pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated PPP4C concentrations in Rabbit serum, plasma and other body fluids. Using Purified Rabbit PPP4C antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PPP4C and PPP4C antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PPP4C in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for PPP4C elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated PPP4C concentrations in Rabbit serum, plasma and other body fluids. Using Purified Rabbit PPP4C antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PPP4C and PPP4C antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PPP4C in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 126722735
• NCBI GeneID: 100009163
• NCBI Accession #: NP_001075792.1
• NCBI GenBank Nucleotide #: NM_001082323.1
• UniProt Accession #: P11084
• Molecular Weight: 35,037 Da
• NCBI Official Full Name: serine/threonine-protein phosphatase 4 catalytic subunit
• NCBI Official Symbol: PPP4C
• NCBI Official Synonym Symbols: PPX
• NCBI Protein Information: serine/threonine-protein phosphatase 4 catalytic subunit; pp4; PP-X; PP4C; protein phosphatase X; protein phosphatase 4 (formerly X), catalytic subunit
• UniProt Protein Name: Serine/threonine-protein phosphatase 4 catalytic subunit
• UniProt Gene Name: PPP4C
• UniProt Synonym Gene Names: PP4C; Pp4; PP-X
• UniProt Entry Name: PP4C_RABIT

Uniprot Description

Function: Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin

Catalytic activity: [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor: Binds 2 manganese ions per subunit