Mouse mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli) ELISA Kit | MSH2 elisa kit
Mouse DNA mismatch repair protein Msh2, MSH2 ELISA Kit
NCBI and Uniprot Product Information
Uniprot Description
MSH2: Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2- MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis. Heterodimer consisting of MSH2-MSH6 (MutS alpha) or MSH2- MSH3 (MutS beta). Both heterodimer form a ternary complex with MutL alpha (MLH1-PMS1). Interacts with EXO1. Part of the BRCA1- associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with ATR. Interacts with SLX4/BTBD12; this interaction is direct and links MutS beta to SLX4, a subunit of different structure-specific endonucleases. Interacts with SMARCAD1. Ubiquitously expressed. Belongs to the DNA mismatch repair MutS family.
Protein type: Tumor suppressor; DNA-binding
Cellular Component: nuclear chromosome; membrane; MutSalpha complex; intracellular; nucleus; MutSbeta complex
Molecular Function: protein homodimerization activity; dinucleotide repeat insertion binding; single thymine insertion binding; ATPase activity; magnesium ion binding; loop DNA binding; Y-form DNA binding; enzyme binding; four-way junction DNA binding; single guanine insertion binding; guanine/thymine mispair binding; double-stranded DNA binding; ATP binding; single-stranded DNA binding; protein C-terminus binding; DNA-dependent ATPase activity; double-strand/single-strand DNA junction binding; single base insertion or deletion binding; oxidized purine DNA binding; nucleotide binding; ADP binding; protein kinase binding; mismatched DNA binding; centromeric DNA binding; DNA binding; damaged DNA binding; MutLalpha complex binding; dinucleotide insertion or deletion binding
Biological Process: somatic recombination of immunoglobulin genes during immune response; determination of adult life span; maintenance of DNA repeat elements; germ cell development; positive regulation of helicase activity; double-strand break repair; negative regulation of neuron apoptosis; cell cycle arrest; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; response to X-ray; oxidative phosphorylation; negative regulation of DNA recombination; mismatch repair; postreplication repair; in utero embryonic development; male gonad development; somatic hypermutation of immunoglobulin genes; isotype switching; DNA repair; cell cycle; meiotic mismatch repair; response to UV-B; B cell mediated immunity; DNA damage response, signal transduction resulting in induction of apoptosis; intra-S DNA damage checkpoint; B cell differentiation; somatic recombination of immunoglobulin gene segments; meiotic gene conversion; response to DNA damage stimulus; negative regulation of meiotic recombination
Research Articles on MSH2
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Product Notes
The Mouse MSH2 msh2 (Catalog #AAA9317113) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9317113 ELISA Kit recognizes Mouse MSH2. It is sometimes possible for the material contained within the vial of "mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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