Mouse SMAD specific E3 ubiquitin protein ligase 1 ELISA Kit | SMURF1 elisa kit

Mouse E3 ubiquitin-protein ligase SMURF1, SMURF1 ELISA Kit

Cat. Num. AAA926554

• Gene Names: Smurf1; mKIAA1625; 4930431E10Rik
• Reactivity: Mouse
• Synonyms: SMAD specific E3 ubiquitin protein ligase 1; Mouse E3 ubiquitin-protein ligase SMURF1; SMURF1 ELISA Kit; Mouse E3 ubiquitin-protein ligase SMURF1 (SMURF1) ELISA kit; KIAA1625; E3 ubiquitin ligase SMURF1; Smad ubiquitination regulatory factor 1; Smad-specific E3 ubiquitin ligase 1; SMURF1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse SMURF1. No significant cross-reactivity or interference between mouse SMURF1 and analogues was observed.
• Sequence Length: 731

• Samples: Serum, plasma, tissue homogenates, cell lysates
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25 pg/ml-2000 pg/ml
• Sensitivity: < 7.8 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SMURF1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SMURF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SMURF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SMURF1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SMURF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 87044890
• NCBI GeneID: 75788
• NCBI Accession #: NP_001033716.1
• NCBI GenBank Nucleotide #: NM_001038627.1
• UniProt Accession #: Q9CUN6; Q3U412; Q8K300
• Molecular Weight: 83,356 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase SMURF1 isoform 1
• NCBI Official Synonym Full Names: SMAD specific E3 ubiquitin protein ligase 1
• NCBI Official Symbol: Smurf1
• NCBI Official Synonym Symbols: mKIAA1625; 4930431E10Rik
• NCBI Protein Information: E3 ubiquitin-protein ligase SMURF1; Smad ubiquitination regulatory factor 1; SMAD-specific E3 ubiquitin-protein ligase 1
• UniProt Protein Name: E3 ubiquitin-protein ligase SMURF1
• UniProt Gene Name: Smurf1
• UniProt Entry Name: SMUF1_MOUSE

Uniprot Description

SMURF1: E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Interacts with TRAF4. Interacts (via HECT domain) with FBXL15 (via LRR repeats). Interacts with SMAD7 and TGFBR1; SMAD7 recruits SMURF1 to TGFBR1 and regulates TGF-beta receptor degradation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.19; Ubiquitin conjugating system; Ligase; Ubiquitin ligase; EC 6.3.2.-

Cellular Component: membrane; cell soma; axon; cytoplasm; plasma membrane; nucleus

Molecular Function: coenzyme F420-2 alpha-glutamyl ligase activity; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity; ribosomal S6-glutamic acid ligase activity; ubiquitin-protein ligase activity; coenzyme F420-0 gamma-glutamyl ligase activity; ligase activity

Biological Process: BMP signaling pathway; proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; receptor catabolic process; transforming growth factor beta receptor signaling pathway; protein ubiquitination during ubiquitin-dependent protein catabolic process; negative regulation of ossification; protein ubiquitination; protein export from nucleus; ubiquitin-dependent SMAD protein catabolic process; negative regulation of transforming growth factor beta receptor signaling pathway; cell differentiation