Human tumor protein, translationally-controlled 1 ELISA Kit | TPT1 elisa kit

Human Translationally-controlled tumor protein, TPT1 ELISA Kit

Cat. Num. AAA924430

• Gene Names: TPT1; HRF; p02; p23; TCTP
• Reactivity: Human
• Synonyms: tumor protein; translationally-controlled 1; Human Translationally-controlled tumor protein; TPT1 ELISA Kit; Human Translationally-controlled tumor protein (TPT1) ELISA kit; FLJ27337; HRF; TCTP; p02; fortilin; histamine-releasing factor; TPT1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human TPT1. No significant cross-reactivity or interference between human TPT1 and analogues was observed.
• Sequence Length: 172

• Samples: Serum, plasma, tissue homogenates, cell lysates
• Assay Type: Quantitative Sandwich
• Detection Range: 12.5 pg/ml -800 pg/ml
• Sensitivity: Typically less than 3.12 pg/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TPT1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TPT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TPT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPT1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 555943820
• NCBI GeneID: 7178
• NCBI Accession #: NP_001273202.1
• NCBI GenBank Nucleotide #: NM_001286273.1
• UniProt Accession #: P13693; Q6YLS2; Q7Z4J4; Q8TBK7; Q96EE2; Q9UC70; B2R7E5
• Molecular Weight: 19,595 Da
• NCBI Official Full Name: translationally-controlled tumor protein isoform 3
• NCBI Official Synonym Full Names: tumor protein, translationally-controlled 1
• NCBI Official Symbol: TPT1
• NCBI Official Synonym Symbols: HRF; p02; p23; TCTP
• NCBI Protein Information: translationally-controlled tumor protein; fortilin; histamine-releasing factor
• UniProt Protein Name: Translationally-controlled tumor protein
• Protein Family: tRNA 2'-phosphotransferase
• UniProt Gene Name: TPT1
• UniProt Synonym Gene Names: TCTP; HRF
• UniProt Entry Name: TCTP_HUMAN

Uniprot Description

TPT1: a novel heat shock protein with antiapoptotic and chaperone-like activity. Its levels are highly regulated in response to various cellular stimuli and stresses. Downregulated in response to proapoptotic treatments that induce Ca(++) stress, in a PKR-dependent manner. Colocalizes with CHFR to the mitotic spindle. Appears to be involved in calcium binding and microtubule stabilization. Its exosomal secretion is induced following DNA damage. Interacts with MCL1 and STEAP3, a p53-inducible transmembrane protein that facilitates the exosomal secretion of TPT1. Secreted TPT1 acts as a histamine-releasing factor, participates in inflammatory responses by inducing the release of histamine, and may play a role in tumor reversion. May play a direct role in placental calcium transport. Phosphorylation by Plk may decrease its microtubule-stabilizing activity and promote microtubule dynamics following metaphase. Critical for peripheral T cell maintenance and TCR-mediated cell proliferation.

Protein type: Calcium-binding; Microtubule-binding

Chromosomal Location of Human Ortholog: 13q14

Cellular Component: multivesicular body; extracellular space; tubulin complex; cytoplasm; nucleus

Molecular Function: protein binding; calcium ion binding

Biological Process: cellular calcium ion homeostasis; regulation of apoptosis; response to virus; calcium ion transport; negative regulation of apoptosis