Mouse BHLH3 Monoclonal Antibody | anti-BHLH3 antibody

BHLH3 Antibody (N-term)

Cat. Num. AAA9232291

• Gene Names: BHLHE41; DEC2; hDEC2; BHLHB3; SHARP1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Synonyms: BHLH3; Monoclonal Antibody; BHLH3 Antibody (N-term); Purified Mouse Monoclonal Antibody (Mab); Class E basic helix-loop-helix protein 41; bHLHe41; Class B basic helix-loop-helix protein 3; bHLHb3; Differentially expressed in chondrocytes protein 2; hDEC2; Enhancer-of-split and hairy-related protein 1; SHARP-1; BHLHE41; BHLHB3; DEC2; SHARP1; anti-BHLH3 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2b,kappa
• Clone Number: 2135CT82.1.84
• Specificity: This BHLH3 antibody is generated from a mouse immunized with a recombinant protein of human BHLH3.
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

• Applicable Applications for anti-BHLH3 antibody: Western Blot (WB)
• Application Notes: WB: 1:4000
• Antigen Source: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-BHLH3 Antibody (N-term) at 1:4000 dilutionLane 1: HeLa whole cell lysateLane 2: RD whole cell lysateLane 3: SH-SY5Y whole cell lysateLane 4: HepG2 whole cell lysateLane 5: Mouse brain tissue lysateLane 6: Mouse skeletal muscle tissue lysateLane 7: Rat skeletal muscle tissue lysateLane 8: Rat brain tissue lysateLane 9: CHO whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-BHLH3 antibody

Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA/B/G, NR1H3/LXRA, NR1H4 and VDR transactivation activity.

Product Categories/Family for anti-BHLH3 antibody

Crown Antibodies; Primary Antibodies; Cancer; Neuroscience

References

Fujimoto K.,et al.Biochem. Biophys. Res. Commun. 280:164-171(2001).Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.Garriga-Canut M.,et al.J. Biol. Chem. 276:14821-14828(2001).Kawamoto T.,et al.Biochem. Biophys. Res. Commun. 313:117-124(2004).Li Y.,et al.Biochem. J. 382:895-904(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 13540521
• NCBI GeneID: 79365
• NCBI Accession #: NP_110389.1
• NCBI GenBank Nucleotide #: NM_030762.2
• UniProt Accession #: Q9C0J9; A2I2N8
• Molecular Weight: 50,498 Da
• NCBI Official Full Name: class E basic helix-loop-helix protein 41
• NCBI Official Synonym Full Names: basic helix-loop-helix family, member e41
• NCBI Official Symbol: BHLHE41
• NCBI Official Synonym Symbols: DEC2; hDEC2; BHLHB3; SHARP1
• NCBI Protein Information: class E basic helix-loop-helix protein 41; enhancer-of-split and hairy-related protein 1; differentially expressed in chondrocytes protein 2; basic helix-loop-helix domain containing, class B, 3
• UniProt Protein Name: Class E basic helix-loop-helix protein 41
• Protein Family: Transcription factor
• UniProt Gene Name: BHLHE41
• UniProt Synonym Gene Names: BHLHB3; DEC2; SHARP1; bHLHe41; bHLHb3; hDEC2; SHARP-1
• UniProt Entry Name: BHE41_HUMAN

NCBI Description

This gene encodes a transcription factor that belongs to the Hairy/Enhancer of Split subfamily of basic helix-loop-helix factors. The encoded protein functions as a transcriptional repressor and as a regulator of molecular clock. Defects in this gene are associated with the short sleep phenotype. [provided by RefSeq, May 2010]

Uniprot Description

BHLHB3: May be a transcriptional repressor that represses both basal and activated transcription. Homodimerize.

Chromosomal Location of Human Ortholog: 12p12.1

Cellular Component: nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; MRF binding; protein binding; protein homodimerization activity; protein heterodimerization activity; histone deacetylase binding; bHLH transcription factor binding; transcription corepressor activity

Biological Process: circadian rhythm; transcription from RNA polymerase II promoter; cell proliferation; organ morphogenesis; negative regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; negative regulation of transcription, DNA-dependent; cell differentiation

Disease: Short Sleeper