Mouse anti-Human LEF1 Monoclonal Antibody | anti-LEF1 antibody

LEF1 Antibody

Cat. Num. AAA9231242

• Gene Names: LEF1; LEF-1; TCF10; TCF7L3; TCF1ALPHA
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified through a protein G column, followed by dialysis against PBS.
• Synonyms: LEF1; Monoclonal Antibody; LEF1 Antibody; Purified Mouse Monoclonal Antibody (Mab); Lymphoid enhancer-binding factor 1; LEF-1; T cell-specific transcription factor 1-alpha; TCF1-alpha; anti-LEF1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,kappa
• Clone Number: 1866CT561.78.3
• Specificity: This antibody is generated from a mouse immunized with a recombinant protein between 95-283 amino acids from human.
• Purity/Purification: Purified through a protein G column, followed by dialysis against PBS.
• Form/Format: Supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Length: 371

• Applicable Applications for anti-LEF1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:4000
• Antigen Type: Synthesized Peptide
• Antigen Source: Human
• Tissue Location: Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines
• Cellular Location: Nucleus {ECO:0000255|PROSITE- ProRule:PRU00267}. Note=Found in nuclear bodies upon PIASG binding.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks.; For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-LEF1 at dilutionLane 1: SW480 whole cell lysateLane 2: Jurkat whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-LEF1 antibody

Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1- independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.

Product Categories/Family for anti-LEF1 antibody

Crown Antibodies; Primary Antibodies; Cancer; Developmental Biology; Stem Cells

References

Waterman M.L.,et al.Genes Dev. 5:656-669(1991). Hovanes K.,et al.Nucleic Acids Res. 28:1994-2003(2000). Jesse S.,et al.Int. J. Cancer 126:1109-1120(2010). Kobielak A.,et al. Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases. Ota T.,et al.Nat. Genet. 36:40-45(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 195222732
• NCBI GeneID: 51176
• NCBI Accession #: NP_001124185.1
• NCBI GenBank Nucleotide #: NM_001130713.2
• UniProt Accession #: Q9UJU2; Q3ZCU4; Q9HAZ0; B4DG38; B7Z8E2; E9PDK3
• Molecular Weight: 44201
• NCBI Official Full Name: lymphoid enhancer-binding factor 1 isoform 2
• NCBI Official Synonym Full Names: lymphoid enhancer binding factor 1
• NCBI Official Symbol: LEF1
• NCBI Official Synonym Symbols: LEF-1; TCF10; TCF7L3; TCF1ALPHA
• NCBI Protein Information: lymphoid enhancer-binding factor 1
• UniProt Protein Name: Lymphoid enhancer-binding factor 1
• Protein Family: Lymphoid enhancer-binding factor
• UniProt Gene Name: LEF1
• UniProt Synonym Gene Names: LEF-1; TCF1-alpha

NCBI Description

This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

Uniprot Description

Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.