NUP50 blocking peptide

NUP50 Blocking Peptide (C-term)

• Gene Names: NUP50; NPAP60; NPAP60L
• Synonyms: NUP50; NUP50 Blocking Peptide (C-term); Nuclear pore complex protein Nup50; 50 kDa nucleoporin; Nuclear pore-associated protein 60 kDa-like; Nucleoporin Nup50; NPAP60L; NUP50 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human NUP50. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder.
• Sequence Length: 468

• Reconstitution: Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Cellular Location: Nucleus, nuclear pore complex. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side. Note=Localizes to the nucleoplasmic fibrils of the nuclear pore complex (By similarity). Dissociates from the NPC structure early during prophase of mitosis. Associates to the newly formed nuclear membrane during telophase.
• Peptide ID: 5082260
• Tissue Location: Ubiquitous. Highest levels in testis, peripheral blood leukocytes and fetal liver
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for NUP50 blocking peptide

Component of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling. Interacts with multiple transport receptor proteins including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B.

NCBI and Uniprot Product Information

• NCBI GI #: 24497451
• NCBI GeneID: 10762
• NCBI Accession #: NP_009103.2
• NCBI GenBank Nucleotide #: NM_007172.3
• UniProt Accession #: Q9UKX7; O75644; Q8N6V5; Q9NPM9; Q9NPR6; Q9P1K5; B1AHA4; B2RB15
• Molecular Weight: 46,863 Da
• NCBI Official Full Name: nuclear pore complex protein Nup50 isoform b
• NCBI Official Synonym Full Names: nucleoporin 50
• NCBI Official Symbol: NUP50
• NCBI Official Synonym Symbols: NPAP60; NPAP60L
• NCBI Protein Information: nuclear pore complex protein Nup50
• UniProt Protein Name: Nuclear pore complex protein Nup50
• Protein Family: Nuclear pore complex protein
• UniProt Gene Name: NUP50
• UniProt Synonym Gene Names: NPAP60L

NCBI Description

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. Pseudogenes of this gene are found on chromosomes 5, 6, and 14. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

NUP50: Component of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin- alpha:beta-cargo complex and importin recycling. Interacts with multiple transport receptor proteins including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear import; Nucleoporin

Chromosomal Location of Human Ortholog: 22q13.31

Cellular Component: cytoplasm; nuclear membrane; nucleoplasm

Molecular Function: protein binding; Ran GTPase binding

Biological Process: mitotic nuclear envelope disassembly; mRNA export from nucleus; protein import into nucleus; protein sumoylation; RNA-mediated gene silencing; tRNA export from nucleus; viral reproduction; viral transcription