lin28a blocking peptide

lin28a Blocking Peptide (Center)

• Gene Names: lin28a; lin28; sb:cb650; zgc:55584
• Synonyms: lin28a; lin28a Blocking Peptide (Center); Protein lin-28 homolog A; Lin-28A; lin28; lin28a blocking peptide

• Specificity: The synthetic peptide sequence is selected from aa 85-99 of HUMAN lin28a
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 202

• Cellular Location: Cytoplasm.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for lin28a blocking peptide

Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting an uridylyltransferase, leading to the terminal uridylation of pre- let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 82241857
• NCBI GeneID: 394066
• UniProt Accession #: Q803L0; Q1LXL3
• Molecular Weight: 21,886 Da
• NCBI Official Full Name: Protein lin-28 homolog A
• NCBI Official Synonym Full Names: lin-28 homolog A (C. elegans)
• NCBI Official Symbol: lin28a
• NCBI Official Synonym Symbols: lin28; sb:cb650; zgc:55584
• NCBI Protein Information: protein lin-28 homolog A
• UniProt Protein Name: Protein lin-28 homolog A
• Protein Family: Protein
• UniProt Gene Name: lin28a
• UniProt Synonym Gene Names: lin28; Lin-28A
• UniProt Entry Name: LN28A_DANRE

Uniprot Description

RNA-binding protein that inhibits processing of pre-let-7 miRNAs and regulates translation of mRNAs that control developmental timing, pluripotency and metabolism. Seems to recognize a common structural G-quartet (G4) feature in its miRNA and mRNA targets (). 'Translational enhancer' that drives specific mRNAs to polysomes and increases the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in mRNA stabilization. Suppressor of microRNA (miRNA) biogenesis, including that of let-7. Binds specific target miRNA precursors (pre-miRNAs), recognizing an 5'-GGAG-3' motif found in their terminal loop, and recruits uridylyltransferase. This results in the terminal uridylation of target pre-miRNAs. Uridylated pre-miRNAs fail to be processed by Dicer and undergo degradation (). Localized to the periendoplasmic reticulum area, binds to a large number of spliced mRNAs and inhibits the translation of mRNAs destined for the ER, reducing the synthesis of transmembrane proteins, ER or Golgi lumen proteins, and secretory proteins. Binds to and enhances the translation of mRNAs for several metabolic enzymes, increasing glycolysis and oxidative phosphorylation. Which, with the let-7 repression may enhance tissue repair in adult tissue ().