Melk blocking peptide

Melk Blocking Peptide (Center)

• Gene Names: Melk; MPK38; AI327312; mKIAA0175
• Synonyms: Melk; Melk Blocking Peptide (Center); Maternal embryonic leucine zipper kinase; Protein kinase PK38; mPK38; Tyrosine-protein kinase MELK; Kiaa0175; Pk38; Melk blocking peptide

• Specificity: The synthetic peptide sequence is selected from aa 381-395 of HUMAN Melk
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 643

• Cellular Location: Cell membrane; Peripheral membrane protein
• Tissue Location: Expressed in testis, ovary, thymus, spleen and T-cell. Expressed by neural progenitors: highly enriched in cultures containing multipotent progenitors
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for Melk blocking peptide

Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.

NCBI and Uniprot Product Information

• NCBI GI #: 341940950
• NCBI GeneID: 17279
• UniProt Accession #: Q61846; Q3TPU1; Q61804; Q6ZQH6
• Molecular Weight: 72,729 Da
• NCBI Official Full Name: Maternal embryonic leucine zipper kinase
• NCBI Official Synonym Full Names: maternal embryonic leucine zipper kinase
• NCBI Official Symbol: Melk
• NCBI Official Synonym Symbols: MPK38; AI327312; mKIAA0175
• NCBI Protein Information: maternal embryonic leucine zipper kinase
• UniProt Protein Name: Maternal embryonic leucine zipper kinase
• Protein Family: Maternal embryonic leucine zipper kinase
• UniProt Gene Name: Melk
• UniProt Synonym Gene Names: Kiaa0175; Pk38; mPK38
• UniProt Entry Name: MELK_MOUSE

Uniprot Description

MELK: Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis. Monomer. Interacts with ZNF622 and PPP1R8. Up-regulated in many cancers cells. Up-regulated upon treatment with radiation or 5-fluorouracil (5-FU) in colorectal cancer cells, suggesting that it might be associated with increased resistance of colorectal cells against radiation and 5- FU. Down-regulated upon siomycin A, a thiazole antibiotic, treatment, leading to inhibit tumor growth in vivo. Expressed in placenta, kidney, thymus, testis, ovary and intestine. Activated by autophosphorylation of the T-loop at Thr-167 and Ser-171: in contrast to other members of the SNF1 subfamily, phosphorylation at Thr-167 is not mediated by STK11/LKB1 but via autophosphorylation instead. Inhibited by calcium-binding. Kinase activity is also regulated by reducing agents: dithiothreitol (DTT) or reduced glutathione are required for kinase activity in vitro; such dependence is however not due to the presence of disulfide bonds. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.

Protein type: EC 2.7.10.2; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; Kinase, protein; RNA splicing; EC 2.7.11.1; CAMK group; CAMKL family; MELK subfamily

Cellular Component: cell cortex; cytoplasm; membrane; nucleus; plasma membrane

Molecular Function: calcium ion binding; non-membrane spanning protein tyrosine kinase activity; protein binding; protein kinase activity; protein serine/threonine kinase activity

Biological Process: apoptosis; cell proliferation; induction of apoptosis by oxidative stress; peptidyl-tyrosine phosphorylation; positive regulation of apoptosis; protein amino acid autophosphorylation