CHMP3 blocking peptide

CHMP3 Blocking Peptide (N-term)

• Gene Names: RNF103-CHMP3; RNF103-VPS24
• Synonyms: CHMP3; CHMP3 Blocking Peptide (N-term); Charged multivesicular body protein 3; Chromatin-modifying protein 3; Neuroendocrine differentiation factor; Vacuolar protein sorting-associated protein 24; hVps24; CGI149; NEDF; VPS24; CHMP3 blocking peptide

• Specificity: The synthetic peptide sequence is selected from aa 28-41 of HUMAN CHMP3
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 222

• Cellular Location: Cytoplasm, cytosol. Membrane; Lipid-anchor. Endosome. Late endosome membrane. Note: Localizes to the midbody of dividing cells
• Tissue Location: Widely expressed. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for CHMP3 blocking peptide

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Selectively binds to phosphatidylinositol 3,5-bisphosphate PtdIns(3,5)P2 and PtdIns(3,4)P2 in preference to other phosphoinositides tested. Involved in late stages of cytokinesis. Plays a role in endosomal sorting/trafficking of EGF receptor. Isoform 2 prevents stress- mediated cell death and accumulation of reactive oxygen species when expressed in yeast cells.

NCBI and Uniprot Product Information

• NCBI GI #: 73917763
• NCBI GeneID: 100526767
• UniProt Accession #: Q9Y3E7; Q3ZTS9; Q53S71; Q53SU5; Q9NZ51; A8K3W0; B4DG34; B8ZZM0; B8ZZX5
• Molecular Weight: 20,770 Da
• NCBI Official Full Name: Charged multivesicular body protein 3
• NCBI Official Synonym Full Names: RNF103-CHMP3 readthrough
• NCBI Official Symbol: RNF103-CHMP3
• NCBI Official Synonym Symbols: RNF103-VPS24
• NCBI Protein Information: RNF103-CHMP3 protein
• UniProt Protein Name: Charged multivesicular body protein 3
• Protein Family: Charged multivesicular body protein
• UniProt Gene Name: CHMP3
• UniProt Synonym Gene Names: CGI149; NEDF; VPS24; hVps24
• UniProt Entry Name: CHMP3_HUMAN

NCBI Description

This locus represents naturally occurring read-through transcription between the neighboring RNF103 (ring finger protein 103) and CHMP3 (charged multivesicular body protein 3) genes. The read-through transcript encodes a fusion protein that shares sequence identity with each individual gene product. [provided by RefSeq, Oct 2011]

Uniprot Description

VPS24: Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Selectively binds to phosphatidylinositol 3,5-bisphosphate PtdIns(3,5)P2 and PtdIns(3,4)P2 in preference to other phosphoinositides tested. Involved in late stages of cytokinesis. Plays a role in endosomal sorting/trafficking of EGF receptor. Isoform 2 prevents stress- mediated cell death and accumulation of reactive oxygen species when expressed in yeast cells. Belongs to the SNF7 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 2p11.2

Cellular Component: cytoplasmic membrane-bound vesicle; cytosol; plasma membrane

Molecular Function: phosphatidylcholine binding; protein binding; protein homodimerization activity

Biological Process: autophagy; cell separation during cytokinesis; endosome transport; mitotic metaphase plate congression; nuclear organization and biogenesis; protein heterooligomerization; protein polymerization; regulation of viral reproduction; viral infectious cycle