EPHA2 blocking peptide

EPHA2 Blocking Peptide (Center)

• Gene Names: EPHA2; ECK; CTPA; ARCC2; CTPP1; CTRCT6
• Synonyms: EPHA2; EPHA2 Blocking Peptide (Center); Ephrin type-A receptor 2; Epithelial cell kinase; Tyrosine-protein kinase receptor ECK; ECK; EPHA2 blocking peptide

• Specificity: The synthetic peptide sequence is selected from aa 518-532 of HUMAN EPHA2
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 976

• Cellular Location: Cell membrane; Single-pass type I membrane protein. Cell projection, ruffle membrane; Single-pass type I membrane protein. Cell projection, lamellipodium membrane; Single- pass type I membrane protein. Cell junction, focal adhesion Note: Present at regions of cell-cell contacts but also at the leading edge of migrating cells
• Tissue Location: Expressed in brain and glioma tissue and glioma cell lines (at protein level). Expressed most highly in tissues that contain a high proportion of epithelial cells, e.g skin, intestine, lung, and ovary.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for EPHA2 blocking peptide

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand- independent stimulatory effect on chemotactic cell migration. During development, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Involved for instance in angiogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 229462861
• NCBI GeneID: 1969
• UniProt Accession #: P29317; Q8N3Z2; B5A968
• Molecular Weight: 54,305 Da
• NCBI Official Full Name: Ephrin type-A receptor 2
• NCBI Official Synonym Full Names: EPH receptor A2
• NCBI Official Symbol: EPHA2
• NCBI Official Synonym Symbols: ECK; CTPA; ARCC2; CTPP1; CTRCT6
• NCBI Protein Information: ephrin type-A receptor 2
• UniProt Protein Name: Ephrin type-A receptor 2
• Protein Family: Ephrin type-A receptor
• UniProt Gene Name: EPHA2
• UniProt Synonym Gene Names: ECK
• UniProt Entry Name: EPHA2_HUMAN

NCBI Description

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders.[provided by RefSeq, May 2010]

Uniprot Description

EphA2: a receptor tyrosine kinase. Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A3, -A4 AND -A5. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. Overexpressed in many cancers including aggressive ovarian, cervical and breast carcinomas, and lung cancer. Expression correlates with degree of angiogenesis, metastasis and xenograft tumor growth. Soluble receptor inhibits tumor growth and angiogenesis in mice.

Protein type: Protein kinase, TK; Protein kinase, tyrosine (receptor); Membrane protein, integral; EC 2.7.10.1; Kinase, protein; TK group; Eph family

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: cell surface; focal adhesion; integral to plasma membrane; plasma membrane

Molecular Function: protein binding; transmembrane receptor protein tyrosine kinase activity

Biological Process: bone remodeling; cell migration; DNA damage response, signal transduction resulting in induction of apoptosis; ephrin receptor signaling pathway; keratinocyte differentiation; mammary gland epithelial cell proliferation; multicellular organismal development; negative regulation of protein kinase B signaling cascade; osteoblast differentiation; osteoclast differentiation; protein kinase B signaling cascade; regulation of angiogenesis; regulation of blood vessel endothelial cell migration; regulation of cell adhesion mediated by integrin

Disease: Cataract 6, Multiple Types