CTNNB1 blocking peptide

CTNNB1 Blocking Peptide (C-term)

• Gene Names: CTNNB1; CTNNB; MRD19; armadillo
• Synonyms: CTNNB1; CTNNB1 Blocking Peptide (C-term); Catenin beta-1; Beta-catenin; CTNNB; CTNNB1 blocking peptide

• Specificity: The synthetic peptide sequence is selected from aa 736-749 of HUMAN CTNNB1
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 781

• Cellular Location: Cytoplasm. Nucleus. Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell junction. Cell membrane. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Note: Colocalized with RAPGEF2 and TJP1 at cell-cell contacts (By similarity). Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1 Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization The majority of beta-catenin is localized to the cell membrane. In interphase, colocalizes with CROCC between CEP250 puncta at the proximal end of centrioles, and this localization is dependent on CROCC and CEP250. In mitosis, when NEK2 activity increases, it localizes to centrosomes at spindle poles independent of CROCC Colocalizes with CDK5 in the cell-cell contacts and plasma membrane of undifferentiated and differentiated neuroblastoma cells.
• Tissue Location: Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. Present in cortical neurons (at protein level).
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for CTNNB1 blocking peptide

Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22647378, PubMed:22699938, PubMed:22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 461854
• NCBI GeneID: 1499
• UniProt Accession #: P35222; Q8NEW9; Q8NI94; Q9H391; A8K1L7
• Molecular Weight: 9,501 Da
• NCBI Official Full Name: Catenin beta-1
• NCBI Official Synonym Full Names: catenin beta 1
• NCBI Official Symbol: CTNNB1
• NCBI Official Synonym Symbols: CTNNB; MRD19; armadillo
• NCBI Protein Information: catenin beta-1
• UniProt Protein Name: Catenin beta-1
• Protein Family: Catenin
• UniProt Gene Name: CTNNB1
• UniProt Synonym Gene Names: CTNNB
• UniProt Entry Name: CTNB1_HUMAN

NCBI Description

The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Oct 2009]

Uniprot Description

CTNNB1: a regulator of cell adhesion and a key downstream effector in the Wnt signaling pathway. Implicated early embryonic development and tumorigenesis. Phosphorylated and destabilized by CK1 and GSK-3beta. Stabilized cytoplasmic beta-catenin is a hallmark of a variety of cancers. Stabilized beta-catenin translocates to the nucleus, where it acts as a transcriptional activator of T-cell factor (TCF)-regulated genes. Interacts with the PDZ domain of TAX1BP3, inhibiting its transcriptional activity. Two alternatively spliced human isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Cell adhesion; Transcription factor; Oncoprotein; Actin-binding

Chromosomal Location of Human Ortholog: 3p21

Cellular Component: adherens junction; basolateral plasma membrane; beta-catenin destruction complex; catenin complex; cell cortex; cell junction; cell-cell adherens junction; centrosome; cytoplasm; cytosol; focal adhesion; intercellular junction; lateral plasma membrane; membrane; nucleoplasm; nucleus; perinuclear region of cytoplasm; plasma membrane; protein complex; transcription factor complex

Molecular Function: alpha-catenin binding; cadherin binding; enzyme binding; estrogen receptor binding; kinase binding; nuclear hormone receptor binding; protein binding; protein C-terminus binding; protein phosphatase binding; SMAD binding; transcription coactivator activity; transcription factor binding

Biological Process: cell adhesion; cell-cell adhesion; epithelial to mesenchymal transition; hair cell differentiation; negative regulation of cell proliferation; negative regulation of mitotic cell cycle, embryonic; negative regulation of protein sumoylation; negative regulation of transcription, DNA-dependent; patterning of blood vessels; positive regulation of apoptosis; positive regulation of histone H3-K4 methylation; positive regulation of interferon type I production; positive regulation of muscle cell differentiation; positive regulation of neuroblast proliferation; positive regulation of neuron apoptosis; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; proteasomal ubiquitin-dependent protein catabolic process; regulation of angiogenesis; regulation of centriole-centriole cohesion; regulation of fibroblast proliferation; regulation of neurogenesis; regulation of smooth muscle cell proliferation; response to drug; response to estradiol stimulus; stem cell maintenance; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin; Wnt receptor signaling pathway, calcium modulating pathway

Disease: Colorectal Cancer; Hepatocellular Carcinoma; Mental Retardation, Autosomal Dominant 19; Ovarian Cancer; Pilomatrixoma