NCBP1 blocking peptide

NCBP1 Blocking Peptide (C-term)

• Gene Names: NCBP1; NCBP; Sto1; CBP80
• Synonyms: NCBP1; NCBP1 Blocking Peptide (C-term); Nuclear cap-binding protein subunit 1; 80 kDa nuclear cap-binding protein; CBP80; NCBP 80 kDa subunit; NCBP; NCBP1 blocking peptide

• Specificity: The synthetic peptide sequence is selected from aa 662-675 of HUMAN NCBP1
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 790

• Cellular Location: Nucleus. Cytoplasm. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for NCBP1 blocking peptide

Component of the cap-binding complex (CBC), which binds cotranscriptionally to the 5'-cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5'-end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2 and is required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP1/CBP80 does not bind directly capped RNAs (m7GpppG-capped RNA) but is required to stabilize the movement of the N-terminal loop of NCBP2/CBP20 and lock the CBC into a high affinity cap-binding state with the cap structure.

NCBI and Uniprot Product Information

• NCBI GI #: 4505343
• NCBI GeneID: 4686
• NCBI Accession #: NP_002477.1
• NCBI GenBank Nucleotide #: NM_002486.4
• UniProt Accession #: Q09161; Q59G76; Q5T1V0; Q5T7X2; B2R718
• Molecular Weight: 91,839 Da
• NCBI Official Full Name: nuclear cap-binding protein subunit 1
• NCBI Official Synonym Full Names: nuclear cap binding protein subunit 1
• NCBI Official Symbol: NCBP1
• NCBI Official Synonym Symbols: NCBP; Sto1; CBP80
• NCBI Protein Information: nuclear cap-binding protein subunit 1
• UniProt Protein Name: Nuclear cap-binding protein subunit 1
• UniProt Gene Name: NCBP1
• UniProt Synonym Gene Names: CBP80; NCBP; CBP80; NCBP 80 kDa subunit
• UniProt Entry Name: NCBP1_HUMAN

NCBI Description

The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. [provided by RefSeq, Jul 2008]

Uniprot Description

NCBP1: mediates U snRNA export from the nucleus. Binds to 5' capped mRNA. Found in aU snRNA export complex with RNUXA/PHAX, CBP20, CBP80, RAN, XPO1 and m7G-capped RNA. Interaction with RNUXA/PHAX. Heterodimer with NCBP2. Is part of the exon junction complex (EJC) containing NCBP1, NCBP2, RNPS1, RBM8A, SRRM1, NXF1, UPF3B, UPF2, THOC4 and/or REFBP2.

Protein type: RNA processing; Spliceosome; Translation

Chromosomal Location of Human Ortholog: 9q34.1

Cellular Component: cytoplasm; cytosol; mitochondrion; mRNA cap complex; nucleoplasm; nucleus; ribonucleoprotein complex

Molecular Function: protein binding; RNA binding

Biological Process: fibroblast growth factor receptor signaling pathway; gene expression; histone mRNA metabolic process; mRNA 3'-end processing; mRNA capping; mRNA catabolic process, nonsense-mediated decay; mRNA export from nucleus; nuclear export; nuclear mRNA splicing, via spliceosome; positive regulation of cell growth; positive regulation of mRNA 3'-end processing; regulation of translational initiation; RNA elongation from RNA polymerase II promoter; RNA export from nucleus; RNA splicing; snRNA transcription from RNA polymerase II promoter; termination of RNA polymerase II transcription; transcription from RNA polymerase II promoter