TPK1 blocking peptide

TPK1 Antibody (N-term) Blocking Peptide

• Gene Names: TPK1; PP20; HTPK1; THMD5
• Synonyms: TPK1; TPK1 Antibody (N-term) Blocking Peptide; Thiamin pyrophosphokinase 1; hTPK1; Placental protein 20; PP20; Thiamine pyrophosphokinase 1; TPK1 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of human TPK1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 243

• Tissue Location: Detected in heart, kidney, testis, small intestine and peripheral blood leukocytes, and at very low levels in a variety of tissues.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for TPK1 blocking peptide

Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. Can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate.

NCBI and Uniprot Product Information

• NCBI GI #: 44888537
• NCBI GeneID: 27010
• UniProt Accession #: Q9H3S4; Q6NUR5; Q9H602; A8K0T7; D3DWG0; I6L9B8
• Molecular Weight: 13,962 Da
• NCBI Official Full Name: Thiamin pyrophosphokinase 1
• NCBI Official Synonym Full Names: thiamin pyrophosphokinase 1
• NCBI Official Symbol: TPK1
• NCBI Official Synonym Symbols: PP20; HTPK1; THMD5
• NCBI Protein Information: thiamin pyrophosphokinase 1
• UniProt Protein Name: Thiamin pyrophosphokinase 1
• Protein Family: Thiamin pyrophosphokinase
• UniProt Gene Name: TPK1
• UniProt Synonym Gene Names: hTPK1; PP20
• UniProt Entry Name: TPK1_HUMAN

NCBI Description

This gene encodes a protein, that exists as a homodimer, which catalyzes the conversion of thiamine to thiamine pyrophosphate. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

TPK1: Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. Can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate. Defects in TPK1 are the cause of thiamine metabolism dysfunction syndrome type 5, episodic encephalopathy type (THMD5). An autosomal recessive metabolic disorder due to an inborn error of thiamine metabolism. The phenotype is highly variable, but in general, affected individuals have onset in early childhood of acute encephalopathic episodes associated with increased serum and CSF lactate. These episodes result in progressive neurologic dysfunction manifest as gait disturbances, ataxia, dystonia, and spasticity, which in some cases may result in loss of ability to walk. Cognitive function is usually preserved, although mildly delayed development has been reported. These episodes are usually associated with infection and metabolic decompensation. Some patients may have recovery of some neurologic deficits. Belongs to the thiamine pyrophosphokinase family.

Protein type: EC 2.7.6.2; Kinase, other; Cofactor and Vitamin Metabolism - thiamine

Chromosomal Location of Human Ortholog: 7q34-q35

Cellular Component: cytosol

Molecular Function: thiamin diphosphokinase activity

Biological Process: thiamin and derivative metabolic process; thiamin diphosphate biosynthetic process

Disease: Thiamine Metabolism Dysfunction Syndrome 5 (episodic Encephalopathy Type)