BAT1 Blocking Peptide | DDX39B blocking peptide

BAT1 Antibody (C-term) Blocking Peptide

• Gene Names: DDX39B; BAT1; UAP56; D6S81E
• Synonyms: BAT1; BAT1 Antibody (C-term) Blocking Peptide; Spliceosome RNA helicase DDX39B; 56 kDa U2AF65-associated protein; ATP-dependent RNA helicase p47; DEAD box protein UAP56; HLA-B-associated transcript 1 protein; DDX39B; UAP56; DDX39B blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human BAT1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 428

• Cellular Location: Nucleus. Nucleus speckle. Cytoplasm. Note: Can translocate to the cytoplasm in the presence of MX1. TREX complex assembly seems to occur in regions surrounding nuclear speckles known as perispeckles
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for DDX39B blocking peptide

Involved in nuclear export of spliced and unspliced mRNA. Assembling component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription- independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. May undergo several rounds of ATP hydrolysis during assembly of TREX to drive subsequent loading of components such as ALYREF/THOC and CHTOP onto mRNA. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. Also associates with pre-mRNA independent of ALYREF/THOC4 and the THO complex. Involved in the nuclear export of intronless mRNA; the ATP-bound form is proposed to recruit export adapter ALYREF/THOC4 to intronless mRNA; its ATPase activity is cooperatively stimulated by RNA and ALYREF/THOC4 and ATP hydrolysis is thought to trigger the dissociation from RNA to allow the association of ALYREF/THOC4 and the NXF1-NXT1 heterodimer. Involved in transcription elongation and genome stability.

NCBI and Uniprot Product Information

• NCBI GI #: 2500529
• NCBI GeneID: 7919
• UniProt Accession #: Q13838; O43496; Q0EFA1; Q2L6F9; Q53GL9; Q5RJ64; Q5RJ66; Q5ST94; Q5STB4; Q5STB5; Q5STB7; B0S8C0
• Molecular Weight: 50,679 Da
• NCBI Official Full Name: Spliceosome RNA helicase DDX39B
• NCBI Official Synonym Full Names: DEAD-box helicase 39B
• NCBI Official Symbol: DDX39B
• NCBI Official Synonym Symbols: BAT1; UAP56; D6S81E
• NCBI Protein Information: spliceosome RNA helicase DDX39B
• UniProt Protein Name: Spliceosome RNA helicase DDX39B
• Protein Family: Spliceosome RNA helicase
• UniProt Gene Name: DDX39B
• UniProt Synonym Gene Names: BAT1; UAP56
• UniProt Entry Name: DX39B_HUMAN

NCBI Description

This gene encodes a member of the DEAD box family of RNA-dependent ATPases that mediate ATP hydrolysis during pre-mRNA splicing. The encoded protein is an essential splicing factor required for association of U2 small nuclear ribonucleoprotein with pre-mRNA, and it also plays an important role in mRNA export from the nucleus to the cytoplasm. This gene belongs to a cluster of genes localized in the vicinity of the genes encoding tumor necrosis factor alpha and tumor necrosis factor beta. These genes are all within the human major histocompatibility complex class III region. Mutations in this gene may be associated with rheumatoid arthritis. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on both chromosomes 6 and 11. Read-through transcription also occurs between this gene and the upstream ATP6V1G2 (ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G2) gene. [provided by RefSeq, Feb 2011]

Uniprot Description

BAT1: Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between ALYREF/THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and ALYREF/THOC4. Belongs to the DEAD box helicase family. DECD subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Spliceosome; EC 3.6.4.13; RNA splicing; Helicase; RNA-binding

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: cytoplasm; nucleoplasm; nucleus; spliceosome

Molecular Function: ATP-dependent protein binding; ATP-dependent RNA helicase activity; ATPase activity; protein binding; RNA-dependent ATPase activity; U4 snRNA binding; U6 snRNA binding

Biological Process: intronless viral mRNA export from host nucleus; mRNA 3'-end processing; mRNA export from nucleus; nuclear mRNA splicing, via spliceosome; positive regulation of RNA elongation; RNA export from nucleus; RNA secondary structure unwinding; RNA splicing; spliceosome assembly; termination of RNA polymerase II transcription