VPS26A blocking peptide

VPS26A Antibody (Center) Blocking Peptide

• Gene Names: VPS26A; HB58; PEP8A; VPS26; Hbeta58
• Synonyms: VPS26A; VPS26A Antibody (Center) Blocking Peptide; Vacuolar protein sorting-associated protein 26A; Vesicle protein sorting 26A; hVPS26; VPS26; VPS26A blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the Center region of human VPS26A. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 327

• Cellular Location: Cytoplasm. Endosome membrane; Peripheral membrane protein. Early endosome Note: Localizes to tubular profiles adjacent to endosomes (PubMed:15078903). Predominantly found in early not late endosomes (By similarity).
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for VPS26A blocking peptide

Acts as component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. The SNX27-retromer is believed to be involved in endosome-to-plasma membrane trafficking and recycling of a broad spectrum of cargo proteins (Probable). The CSC seems to act as recruitment hub for other proteins, such as the WASH complex and TBC1D5 (Probable). Required for retrograde transport of lysosomal enzyme receptor IGF2R (PubMed:15078902, PubMed:15078903). Required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA) (PubMed:15247922). Required for the endosomal localization of FAM21A (indicative for the WASH complex) (PubMed:22070227). Required for the endosomal localization of TBC1D5 (PubMed:20923837). Mediates retromer cargo reognition of SORL1 and is involved in trafficking of SORL1 implicated in sorting and processing of APP (PubMed:22279231). Involved in retromer- independent lysosomal sorting of F2R (PubMed:16407403). Involved in recycling of ADRB2 (PubMed:21602791). Enhances the affinity of SNX27 for PDZ-binding motifs in cargo proteins (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 25453454
• NCBI GeneID: 9559
• UniProt Accession #: O75436; Q8TBH4; Q9H982; A8MZ56; B2RDD3
• Molecular Weight: 28,938 Da
• NCBI Official Full Name: Vacuolar protein sorting-associated protein 26A
• NCBI Official Synonym Full Names: VPS26, retromer complex component A
• NCBI Official Symbol: VPS26A
• NCBI Official Synonym Symbols: HB58; PEP8A; VPS26; Hbeta58
• NCBI Protein Information: vacuolar protein sorting-associated protein 26A
• UniProt Protein Name: Vacuolar protein sorting-associated protein 26A
• Protein Family: Vacuolar protein sorting-associated protein
• UniProt Gene Name: VPS26A
• UniProt Synonym Gene Names: VPS26; hVPS26
• UniProt Entry Name: VP26A_HUMAN

NCBI Description

This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

VPS26A: Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Belongs to the VPS26 family.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 10q21.1

Cellular Component: cytosol; early endosome; endosome; intracellular membrane-bound organelle; retromer complex; vesicle

Molecular Function: protein binding; protein transporter activity

Biological Process: intracellular protein transport; regulation of macroautophagy; retrograde transport, endosome to Golgi; Wnt receptor signaling pathway