VPS53 blocking peptide

VPS53 Antibody (N-term) Blocking Peptide

• Gene Names: VPS53; HCCS1; PCH2E; hVps53L; pp13624
• Synonyms: VPS53; VPS53 Antibody (N-term) Blocking Peptide; Vacuolar protein sorting-associated protein 53 homolog; VPS53 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of human VPS53. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 699

• Cellular Location: Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for VPS53 blocking peptide

May be involved in retrograde transport of early and late endosomes to the late Golgi. The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD.

NCBI and Uniprot Product Information

• NCBI GI #: 74746777
• NCBI GeneID: 55275
• UniProt Accession #: Q5VIR6; Q8WYW3; Q9BRR2; Q9BY02; Q9NV25; A8K2S8; B3FH42
• Molecular Weight: 94,405 Da
• NCBI Official Full Name: Vacuolar protein sorting-associated protein 53 homolog
• NCBI Official Synonym Full Names: VPS53, GARP complex subunit
• NCBI Official Symbol: VPS53
• NCBI Official Synonym Symbols: HCCS1; PCH2E; hVps53L; pp13624
• NCBI Protein Information: vacuolar protein sorting-associated protein 53 homolog
• UniProt Protein Name: Vacuolar protein sorting-associated protein 53 homolog
• Protein Family: Vacuolar protein sorting-associated protein
• UniProt Gene Name: VPS53
• UniProt Entry Name: VPS53_HUMAN

NCBI Description

This gene encodes a protein with sequence similarity to the yeast Vps53p protein. Vps53p is involved in retrograde vesicle trafficking in late Golgi. [provided by RefSeq, Jul 2008]

Uniprot Description

VPS53: May be involved in retrograde transport of early and late endosomes to the late Golgi. The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD. Belongs to the VPS53 family. 4 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: Golgi apparatus; membrane; perinuclear region of cytoplasm; recycling endosome; trans-Golgi network membrane

Molecular Function: protein binding

Biological Process: endocytic recycling; lysosomal transport; retrograde transport, endosome to Golgi

Disease: Pontocerebellar Hypoplasia, Type 2e