CYP7A1 blocking peptide

CYP7A1 Antibody (C-term) Blocking Peptide

• Gene Names: CYP7A1; CP7A; CYP7; CYPVII
• Synonyms: CYP7A1; CYP7A1 Antibody (C-term) Blocking Peptide; Cholesterol 7-alpha-monooxygenase; CYPVII; Cholesterol 7-alpha-hydroxylase; Cytochrome P450 7A1; CYP7; CYP7A1 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human CYP7A1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 504

• Cellular Location: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein
• Tissue Location: Detected in liver.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for CYP7A1 blocking peptide

Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis.

NCBI and Uniprot Product Information

• NCBI GI #: 544084
• NCBI GeneID: 1581
• UniProt Accession #: P22680; P78454; Q3MIL8; Q7KZ19
• Molecular Weight: 57,661 Da
• NCBI Official Full Name: Cholesterol 7-alpha-monooxygenase
• NCBI Official Synonym Full Names: cytochrome P450 family 7 subfamily A member 1
• NCBI Official Symbol: CYP7A1
• NCBI Official Synonym Symbols: CP7A; CYP7; CYPVII
• NCBI Protein Information: cholesterol 7-alpha-monooxygenase
• UniProt Protein Name: Cholesterol 7-alpha-monooxygenase
• Protein Family: Cholesterol 7-alpha-monooxygenase
• UniProt Gene Name: CYP7A1
• UniProt Synonym Gene Names: CYP7
• UniProt Entry Name: CP7A1_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway in the liver, which converts cholesterol to bile acids. This reaction is the rate limiting step and the major site of regulation of bile acid synthesis, which is the primary mechanism for the removal of cholesterol from the body. Polymorphisms in the promoter of this gene are associated with defects in bile acid synthesis. [provided by RefSeq, Feb 2010]

Uniprot Description

CYP7A1: Catalyzes a rate-limiting step in cholesterol catabolism and bile acid biosynthesis by introducing a hydrophilic moiety at position 7 of cholesterol. Important for cholesterol homeostasis. Belongs to the cytochrome P450 family.

Protein type: EC 1.14.13.17; Oxidoreductase; Endoplasmic reticulum; Lipid Metabolism - primary bile acid biosynthesis

Chromosomal Location of Human Ortholog: 8q11-q12

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle

Molecular Function: cholesterol 7-alpha-monooxygenase activity

Biological Process: bile acid biosynthetic process; cholesterol catabolic process; cholesterol homeostasis; sterol metabolic process