CYP26B1 blocking peptide

CYP26B1 Antibody (C-term) Blocking Peptide

• Gene Names: CYP26B1; RHFCA; CYP26A2; P450RAI2; P450RAI-2
• Synonyms: CYP26B1; CYP26B1 Antibody (C-term) Blocking Peptide; Cytochrome P450 26B1; 114--; Cytochrome P450 26A2; Cytochrome P450 retinoic acid-inactivating 2; Cytochrome P450RAI-2; Retinoic acid-metabolizing cytochrome; CYP26A2; P450RAI2; CYP26B1 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human CYP26B1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 512

• Cellular Location: Endoplasmic reticulum membrane; Peripheral membrane protein Microsome membrane; Peripheral membrane protein
• Tissue Location: Highly expressed in brain, particularly in the cerebellum and pons.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for CYP26B1 blocking peptide

Involved in the metabolism of retinoic acid (RA), rendering this classical morphogen inactive through oxidation. Involved in the specific inactivation of all-trans-retinoic acid (all-trans-RA), with a preference for the following substrates: all-trans-RA > 9-cis-RA > 13-cis-RA. Generates several hydroxylated forms of RA, including 4-OH-RA, 4-oxo-RA, and 18-OH- RA. Esential for postnatal survival. Plays a central role in germ cell development: acts by degrading RA in the developing testis, preventing STRA8 expression, thereby leading to delay of meiosis. Required for the maintenance of the undifferentiated state of male germ cells during embryonic development in Sertoli cells, inducing arrest in G0 phase of the cell cycle and preventing meiotic entry. Plays a role in skeletal development, both at the level of patterning and in the ossification of bone and the establishment of some synovial joints.

NCBI and Uniprot Product Information

• NCBI GI #: 20137526
• NCBI GeneID: 56603
• UniProt Accession #: Q9NR63; Q32MC0; Q53TW1; Q9NP41; B2R8M7; B7Z2K6; B7Z2P4; B7Z3B8; E4W5W7
• Molecular Weight: 55,756 Da
• NCBI Official Full Name: Cytochrome P450 26B1
• NCBI Official Synonym Full Names: cytochrome P450 family 26 subfamily B member 1
• NCBI Official Symbol: CYP26B1
• NCBI Official Synonym Symbols: RHFCA; CYP26A2; P450RAI2; P450RAI-2
• NCBI Protein Information: cytochrome P450 26B1
• UniProt Protein Name: Cytochrome P450 26B1
• Protein Family: Cytochrome
• UniProt Gene Name: CYP26B1
• UniProt Synonym Gene Names: CYP26A2; P450RAI2; Cytochrome P450RAI-2
• UniProt Entry Name: CP26B_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The encoded protein is localized to the endoplasmic reticulum, and functions as a critical regulator of all-trans retinoic acid levels by the specific inactivation of all-trans retinoic acid to hydroxylated forms. Mutations in this gene are associated with radiohumeral fusions and other skeletal and craniofacial anomalies, and increased levels of the encoded protein are associated with atherosclerotic lesions. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2013]

Uniprot Description

CYP26B1: Involved in the metabolism of retinoic acid (RA), rendering this classical morphogen inactive through oxidation. Involved in the specific inactivation of all-trans-retinoic acid (all-trans-RA), with a preference for the following substrates: all-trans-RA > 9-cis-RA > 13-cis-RA. Generates several hydroxylated forms of RA, including 4-OH-RA, 4-oxo-RA, and 18-OH- RA. Esential for postnatal survival. Plays a central role in germ cell development: acts by degrading RA in the developing testis, preventing STRA8 expression, thereby leading to delay of meiosis. Required for the maintenance of the undifferentiated state of male germ cells during embryonic development in Sertoli cells, inducing arrest in G0 phase of the cell cycle and preventing meiotic entry. Plays a role in skeletal development, both at the level of patterning and in the ossification of bone and the establishment of some synovial joints. Defects in CYP26B1 are the cause of radiohumeral fusions with other skeletal and craniofacial anomalies (RHFCA). A disease characterized by craniofacial malformations, occipital encephalocele, radiohumeral fusions, oligodactyly, advanced osseous maturation, and calvarial mineralization defects. Belongs to the cytochrome P450 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.14.-.-; Cofactor and Vitamin Metabolism - retinol; Cell development/differentiation; Oxidoreductase

Chromosomal Location of Human Ortholog: 2p13.2

Cellular Component: cytoplasm; endoplasmic reticulum membrane

Molecular Function: retinoic acid 4-hydroxylase activity; retinoic acid binding

Biological Process: cell fate determination; embryonic limb morphogenesis; male meiosis; negative regulation of retinoic acid receptor signaling pathway; proximal/distal pattern formation; spermatogenesis; vitamin metabolic process

Disease: Radiohumeral Fusions With Other Skeletal And Craniofacial Anomalies